April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
The Structural Role Of Pax6 Over-expression In The Formation Of Microcorneas
Author Affiliations & Notes
  • Erin P. Dooley
    School of Optometry and Vision Sciences,
    Cardiff University, Cardiff, United Kingdom
  • Craig Boote
    School of Optometry and Vision Sciences,
    Cardiff University, Cardiff, United Kingdom
  • Natalie Dora
    Reproductive and Developmental Sciences, University of Edinburgh, Edinburgh, United Kingdom
  • John D. West
    Reproductive and Developmental Sciences, University of Edinburgh, Edinburgh, United Kingdom
  • Christina S. Kamma-Lorger
    School of Optometry and Vision Sciences,
    Cardiff University, Cardiff, United Kingdom
  • Andrew J. Quantock
    School of Optometry and Vision Sciences,
    Cardiff University, Cardiff, United Kingdom
  • Keith M. Meek
    Optometry & Vision Sciences,
    Cardiff University, Cardiff, United Kingdom
  • Footnotes
    Commercial Relationships  Erin P. Dooley, None; Craig Boote, None; Natalie Dora, None; John D. West, None; Christina S. Kamma-Lorger, None; Andrew J. Quantock, None; Keith M. Meek, None
  • Footnotes
    Support  MRC Grant G0600755
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1989. doi:
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      Erin P. Dooley, Craig Boote, Natalie Dora, John D. West, Christina S. Kamma-Lorger, Andrew J. Quantock, Keith M. Meek; The Structural Role Of Pax6 Over-expression In The Formation Of Microcorneas. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1989.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To better understand the structural role of Pax6 over-expression in hemizygous PAX77+/- transgenic mice (carrying 5-6 copies of the human PAX6 gene) in the formation of microcorneas.

Methods: : Corneas of CBA-PAX77+/- mice (hemizygous PAX77+/- mice on a CBA/Ca genetic background) and wild-type (CBA/Ca) mice were dissected, preserved in buffered paraformaldehyde and examined by wide angle X-ray scattering at station I02 at the Diamond Light Source (Didcot, UK). The corneas were scanned using a 200 µm x 200 µm beam at a scanning interval of 0.25 mm with a 10 second exposure time. X-ray scatter patterns were recorded at each position, and subsequent data analysis quantified the preferential orientation and distribution of stromal collagen fibrils across the cornea from limbus to limbus.

Results: : PAX77+/- corneas were smaller than the wild-type strain. Differences in the preferential alignment and distribution of stromal collagen fibrils were evident between the PAX77+/- and wild-type strains. Preliminary data showed that in PAX77+/-corneas collagen lamellae were oriented in a unilateral inferior/superior direction throughout, unlike wild-type corneas which showed a more annular arrangement of lamellae.

Conclusions: : Pax6 over-expression in PAX77+/- corneas is associated with changes in collagen lamella arrangement, which may be related to the microcornea phenotype.

Keywords: cornea: basic science • genetics • extracellular matrix 
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