April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Effect Of Plasma Rich In Growth Factors (PRGF) On Corneal Epithelial Wound Healing
Author Affiliations & Notes
  • Eduardo Anitua
    Eduardo Anitua Foundation, Vitoria, Spain
  • Francisco Muruzabal
    Eduardo Anitua Foundation, Vitoria, Spain
  • Maria De la Fuente
    Eduardo Anitua Foundation, Vitoria, Spain
  • Jesús Merayo
    Instituto Oftalmologico Fernandez-Vega, Oviedo, Spain
  • Gorka Orive
    Eduardo Anitua Foundation, Vitoria, Spain
  • Footnotes
    Commercial Relationships  Eduardo Anitua, Pioneer of PRGF technology (P); Francisco Muruzabal, Scientist of BTI (E); Maria De la Fuente, Scientist of BTI (E); Jesús Merayo, None; Gorka Orive, Scientist of BTI (E)
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 1991. doi:
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      Eduardo Anitua, Francisco Muruzabal, Maria De la Fuente, Jesús Merayo, Gorka Orive; Effect Of Plasma Rich In Growth Factors (PRGF) On Corneal Epithelial Wound Healing. Invest. Ophthalmol. Vis. Sci. 2011;52(14):1991.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To evaluate the effects of PRGF on corneal epithelial cell proliferation and wound healing.

Methods: : Blood from healthy donors was collected, centrifuged and, Plasma Rich in Growth Factors (PRGF) was drawn off avoiding the buffy coat. Human corneal epithelial cells (HCE) transfected with SV40 adenivirus were cultured. The proliferation assays was carried out using the cyquant assay after the treatment of cells with PRGF. In order to quantify the wound closure potential of epithelial cells after the treatment of PRGF, cells were plated in culture inserts placed on a 24-well plate at high density. After removing the inserts, cells were treated with PRGF and the wound healing area was photographed each 4 hours until complete closure.

Results: : Proliferation rate of epithelial cells stimulated with PRGF increased significantly when compared with PRGF non-treated group. Results from the in vitro wound healing study revealed that experimental area of 8.1 mm2 was totally closed by the epithelial cells (100% of the damage area) in 24 hours after treatment with PRGF, while the non-treated control group was only partially closed (35% of total area).

Conclusions: : These results suggest that PRGF could accelerate corneal epithelial wound healing.

Keywords: cornea: epithelium • wound healing • growth factors/growth factor receptors 

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