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Marco Marenco, Isabella Mariani, Magda Gharbyia, Stefano Lupo, Serena Fragiotta, Giancarlo Ferrazza, Enzo Maria Vingolo; Combine Use Of Platelet Gel And Autolougus Serum Eye Drop In The Management Of Non-Healing Corneal Ulcer: in vivi confocal microscopy study. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2000.
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to report the use of combined Platlet-gel and autologous serum eye drop in the treatment of persistent corneal ulcers and analyze the effect on corneal tissue by the mean of in vivo confocal microscopy .
11 patients with persistent corneal ulcers not responsive to antibiotic treatment were treated with combined therapy of platelet-gel and autologous serum eyedrops.Best Corrected Visual Acuity, complete eye examination, anterior segment photograph with fluorescein stain and in vivo confocal microscopy were performed and analyzed in all patients for a minimum follow up of 3 months. Preparation: 10 ml of platelet rich plasma (PRP) were obtained from 16 ml of autologous peripheral blood into two REGEN TM RTHT tubes after centrifugation 8 minutes at 1500xg. It’s possible to variate PRP volume transferring in another tube then draw off 2 ml supernatant after centrifugation and homogenize by inversion of the tube. In this way the platelet number can reach twice or three times greater than peripheral values. Platelet gel was performed adding PRP 5 ml of autologous serum plus 2 ml of Ca-gluconate. All patients received topical treatment with antibiotic therapy associated with gel 3 times daily for 3 days followed by topical instillation of autologous serum eye drops 4 times daily until corneal ulcer closure.
in all cases relief of symptoms was obtained and after 20 days closure of corneal ulcer with complete re-epithelialization occurred in all 11 patients. Confocal microscopy performed at resolution of revealed regeneration of nerve plexus with flocculation in all cases.
The presence of plateles together with plasma improve tissues regeneration. Platelet-gel and autologous serum have been used in the treatment of several corneal disorders. Both of them are simple, non invasive and well tolerated and provide a good support for corneal healing in persistent corneal ulcer.
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