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Alan G. Fong, Hyung Cho, Tofik Ali, Swathi Reddy, Barbara Soltz, Robert Soltz, Anurag Shrivastava, Roy S. Chuck; Novel Laser-Activated Biological Glue for Sealing Corneal Wounds. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2024.
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© ARVO (1962-2015); The Authors (2016-present)
To evaluate the effectiveness of a novel laser-activated collagen-flavin bioglue for sealing corneal incisions by measuring bursting pressures after wound closure.
A 2.85mm keratome blade was used to create a non-self-sealing central perpendicular corneal wound in 15 cadaver calf eyes. Each incision was performed by the same surgeon. After drying the corneal wound of residual aqueous humor, the bioglue solder was applied in a strip completely covering the incision. A diode-pumped solid state portable blue light laser (457nm +/-5nm at 450mW) (Conversion Energy Enterprises, Spring Valley, NY) was then used to activate the solder for 3 minutes, resulting in cross-linking to tissue. Wound stability was subsequently tested using a stepwise infusion of basic salt solution at 15mL/hr, and pressure changes were monitored via digital manometer. Bursting pressure was recorded, defined as the first sign of leakage of fluid from the corneal wound.
A total of 15 fresh calf cadaver eyes were used, but only 13 eyes with bursting pressures below 1000mmHg (the maximum limit for digital manometer) were included for analysis. The mean corrected bursting pressure (bursting minus baseline intraocular pressure) after wound closure with the solder was 251.15mmHg (SD 87.53).
The laser-activated collagen-flavin solder was effective in sealing central full-thickness corneal wounds in cadaver calf eyes.
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