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Daisuke Muramatsu, Takeshi Iwase, Brian Oveson, Abdullah Nadri, Sun Young Lee, Tsunehiko Yoshida, Katsuaki Miki, Jude Samulski, Jade Samulski, Peter Campochiaro; Gene Transfer Of Bdnf Promotes Cone Survival And Function In A Model Of Retinitis Pigmentosa (RP). Invest. Ophthalmol. Vis. Sci. 2011;52(14):2051.
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To test the effects of increased expression of BDNF on cone survival in a mouse model of retinitis pigmentosa (RP).
Rd10+/+ or rd10-/- mice in a C57BL/6 background were given a subretinal injection of 5e8 vp of scAAV5-BDNF, scAAV8-BDNF, or vehicle at postnatal day (P) 14. Fourteen days after injection, the levels of Bdnf mRNA were measured in eye cups by real time RT-PCR. At P50, photopic electroretinography (ERG) was done and then mice were euthanized and cone cell density was measured by image analysis of confocal microscopic images of retinal flat mounts stained with rhodamine-labeled peanut agglutinin (PNA).
Compared to eyecups from eyes injected with vehicle, those from eyes injected with scAAV5-BDNF or scAAV8-BDNF showed a 13.5 and 8.5-fold increase in Bdnf mRNA. At P50, mean (±SEM) photopic b-wave amplitude was 24.4±2.7 µV in eyes of rd10+/+ mice injected with scAAV5-BDNF compared to 16.5±1.2 µV in eyes of no treatment controls (p=0.01). Cone density was 126.3±10.0 in eyes of rd10+/+ mice injected with scAAV5-BDNF compared to 92.7±7.8 in no treatment controls (p=0.01).
Gene transfer of Bdnf by subretinal injection of scAAV5-BDNF promotes cone survival and function in a model of RP.
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