April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Regulation Of Cyclic AMP Production In Rat RPE-J Cells By The Synthetic Neuroprostane, BA-12high
Author Affiliations & Notes
  • Catherine A. Opere
    Pharmacy Sciences, Creighton University, Omaha, Nebraska
  • Ya Fatou Njie-Mbye
    Texas Southern University, Houston, Texas
  • Jamal Jamil
    Pharmacy Sciences, Creighton University, Omaha, Nebraska
  • Thierry Durand
    Universities of Montpellier I and II, Institut des Biomolécules Max Mousseron, Montpellier cedex, France
  • Jean-Marie Galano
    Universities of Montpellier I and II, Institut des Biomolécules Max Mousseron, Montpellier cedex, France
  • Alexandre Guy
    Universities of Montpellier I and II, Institut des Biomolécules Max Mousseron, Montpellier cedex, France
  • Madhura S. Kulkarni
    Texas Southern University, Houston, Texas
  • Min Zhao
    Texas Southern University, Houston, Texas
  • Sunny E. Ohia
    Provost/Academic Affairs,
    Texas Southern University, Houston, Texas
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2067. doi:
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      Catherine A. Opere, Ya Fatou Njie-Mbye, Jamal Jamil, Thierry Durand, Jean-Marie Galano, Alexandre Guy, Madhura S. Kulkarni, Min Zhao, Sunny E. Ohia; Regulation Of Cyclic AMP Production In Rat RPE-J Cells By The Synthetic Neuroprostane, BA-12high. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2067.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Neuroprostanes (NeuroPs) are isoprostane-like compounds that are derived from non-enzymatic, free-radical catalyzed peroxidation of docosahexaenoic (22:6 n-3) acid and docosapentaenoic acid (DPA; 22:5 n-6). We have evidence that the synthetic, DPA-derived NP, BA-12high regulates excitatory neurotransmitter release in bovine retina, in vitro (Opere et al., IOVS 46: E-Abstract 3315, 2010). However, the signal transduction processes activated by BA-12high in mammalian retina remain unknown. The present study investigates whether BA-12high regulates cyclic AMP production in rat retinal pigmented epithelial cells (RPE-J).

Methods: : RPE-J cells were exposed to DMEM medium containing cyclooxygenase inhibitor, flurbiprofen (3 µM) and the phosphodiesterase inhibitor isobutylmethylxanthine (2 mM) for 30 minutes prior to treatment with BA12-high or forskolin (positive control) for 20 minutes. After termination of the reaction, cells were lysed and then prepared for cyclic AMP assays using an Enzyme Immunoassay (EIA) kit. Cyclic AMP production was expressed as pmol/mg of protein.

Results: : In the concentration range, 10-9M to 10-4M, BA-12high caused a concentration-dependent increase in cyclic AMP concentrations over basal levels. For instance, BA-12high (10-8M) stimulated cyclic AMP accumulation by 170.5% (n=6; p <.0.05). Furthermore, a maximal stimulatory response of 388.4% (n=6; p < 0.001) was achieved at 1 µM concentration of the NeuroP. The response elicited by forskolin (10 µM) in RPE-J cells was comparable to that of BA-12high (1 µM).

Conclusions: : The synthetic DPA-derived NeuroP, BA-12high can increase cyclic AMP production in rat retinal pigmented epithelial cells. Furthermore, NeuroPs may play a regulatory role in signal transduction processes in mammalian retina.

Keywords: retina • signal transduction • oxidation/oxidative or free radical damage 
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