Abstract
Purpose: :
Lymphangiogenesis in Cornea is induced by various stimuli including infection, inflammation, chemical burn and graft rejection. Angiogenesis and lymphangiogenesis is a common clinical problem. This study was investigated to inhibit lymphangiogenesis by prox1 siRNA. The prox1 is prospero homeobox protein 1 and transcription factor. This protein induces the differentiation of lymphatic endothelial cells.
Methods: :
Induction of lymphatic vessels was stimulated by alkali burning in cornea. 2mm disc with 1N NaOH was applied in central cornea of SD rat for 10 sec. LYVE-1 antibody for lymphatic vessels, and F4/80 antibody for inflammatory cells were used in this study. Prox1 siRNA liposome was treated 2 times per day after burning, topically.
Results: :
The lymphatic vessels were observed in injured cornea by LYVE-1 immunostaing. And the F4/80 positive cells were observed in this cornea. However, lymphatic vessels and F4/80 positive cells were decreased by prox1 siRNA treatment.
Conclusions: :
The prox1 siRNA inhibited lymphangiogenesis and inflammation by chemical burning in cornea. These results suggested that prox1 is a regulatory protein for corneal lymphangiogenesis and this prox1 siRNA should be used for inhibition of corneal lymphangiogenesis.
Keywords: cornea: basic science • inflammation