March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Inhibition of corneal lymphangiogenesis by Prox1 siRNA
Author Affiliations & Notes
  • Jun-Sub Choi
    Ophthalmology & Visual Science, Catholic Univ Korea Coll of Med, Seoul, Republic of Korea
    Catholic Institutes of Visual Science, The Catholic University of Korea, Seoul, Republic of Korea
  • Sun-Young Shin
    Ophthalmology & Visual Science, Catholic Univ Korea Coll of Med, Seoul, Republic of Korea
  • Choun-Ki Joo
    Ophthalmology & Visual Science, Catholic Univ Korea Coll of Med, Seoul, Republic of Korea
    Seoul St. Mary’s hospital Eye Institute (SSEI), The Catholic University of Korea, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  Jun-Sub Choi, None; Sun-Young Shin, None; Choun-Ki Joo, None
  • Footnotes
    Support  Grant of the Korean Health Technology R & D Project, Ministry for Health & Welfare, Republic of Korea (A092258)
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 2386. doi:https://doi.org/
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    • Get Citation

      Jun-Sub Choi, Sun-Young Shin, Choun-Ki Joo; Inhibition of corneal lymphangiogenesis by Prox1 siRNA. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2386. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Lymphangiogenesis in Cornea is induced by various stimuli including infection, inflammation, chemical burn and graft rejection. Angiogenesis and lymphangiogenesis is a common clinical problem. This study was investigated to inhibit lymphangiogenesis by prox1 siRNA. The prox1 is prospero homeobox protein 1 and transcription factor. This protein induces the differentiation of lymphatic endothelial cells.

Methods: : Induction of lymphatic vessels was stimulated by alkali burning in cornea. 2mm disc with 1N NaOH was applied in central cornea of SD rat for 10 sec. LYVE-1 antibody for lymphatic vessels, and F4/80 antibody for inflammatory cells were used in this study. Prox1 siRNA liposome was treated 2 times per day after burning, topically.

Results: : The lymphatic vessels were observed in injured cornea by LYVE-1 immunostaing. And the F4/80 positive cells were observed in this cornea. However, lymphatic vessels and F4/80 positive cells were decreased by prox1 siRNA treatment.

Conclusions: : The prox1 siRNA inhibited lymphangiogenesis and inflammation by chemical burning in cornea. These results suggested that prox1 is a regulatory protein for corneal lymphangiogenesis and this prox1 siRNA should be used for inhibition of corneal lymphangiogenesis.

Keywords: cornea: basic science • inflammation 
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