April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Lacrimal Gland Regeneration By Their Progenitor Cells
Tetsuya Kawakita; Shinya Kobayashi; Motoko Kawashima; Naoko Okada; Masataka Ito; Kenji Mishima; Shigeto Shimmura; Ichiro Saito; Kazuo Tsubota
Author Affiliations & Notes
  • Tetsuya Kawakita
    Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Japan
  • Shinya Kobayashi
    Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Japan
  • Motoko Kawashima
    Department of Ophthalmology, Keio University School of Med, Shinjuku ku, Japan
  • Naoko Okada
    Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Japan
  • Masataka Ito
    Anatomy, National Defense Med College, Tokorozawa-Shi, Japan
  • Kenji Mishima
    Pathology, Tsurumi University, Tsurumi, Japan
  • Shigeto Shimmura
    Ophthalmology, Keio Univ School of Medicine, Tokyo, Japan
  • Ichiro Saito
    Pathology, Tsurumi University, Tsurumi, Japan
  • Kazuo Tsubota
    Ophthalmology, Keio Univ School of Medicine, Shinjuku-ku, Japan
  • Footnotes
    Commercial Relationships  Tetsuya Kawakita, None; Shinya Kobayashi, None; Motoko Kawashima, None; Naoko Okada, None; Masataka Ito, None; Kenji Mishima, None; Shigeto Shimmura, None; Ichiro Saito, None; Kazuo Tsubota, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2092. doi:
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      Tetsuya Kawakita, Shinya Kobayashi, Motoko Kawashima, Naoko Okada, Masataka Ito, Kenji Mishima, Shigeto Shimmura, Ichiro Saito, Kazuo Tsubota; Lacrimal Gland Regeneration By Their Progenitor Cells. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2092.

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Abstract

Purpose: : Previous report showed that mouse lacrimal gland epithelial cells were cultured successfully only in case of newborn mouse lacrimal gland. This study was performed to cultivate and characterize adult mouse lacrimal gland epithelial cells, and investigate regeneration potential of these cells by sphere culture method.

Methods: : Lacrimal glands were removed from newborn mice (C57B/6) and isolated lacrimal gland epithelial cells were seeded on a tissue culture-treated or non-coating dish in Cnt-07 culture medium with or without cholera toxin. Cultivated cells were characterized by pan-cytokeratin, α-smooth muscle actin, and lactoferrin immunostaining. Lacrimal gland cells from 7-weeks old GFP and non-GFP (C57B/6) mice were seeded on non-coating dish or on 3T3 feeder layer for colony forming potential. Sphere generated from newborn lacrimal gland epithelial cells were embedded in collagen and injected into lacrimal gland recession mouse model to evaluate their regeneration potential.

Results: : The lacrimal gland epithelial cells of newborn mice could be selected by cholera toxin, which was confirmed by pan-cytokeratin (+) and α-smooth muscle actin (-). With this medium and low adherent culture dish, adult mice generated sphere formation from single cells. Such cells could also form colony formation on 3T3 feeder cells. Spheres generated from new born lacrimal gland could be transplanted into injured lacrimal gland, and partially recover corneal injured score, but not secretory function.

Conclusions: : Adult mouse lacrimal gland epithelial cells were successfully cultivated and these cells could generate the sphere formation.

Keywords: lacrimal gland • wound healing 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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