April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Generation of Retinal Pigment Epithelial Cells from Human Embryonic Stem Cell-derived Spherical Neural Masses
Sang Jin Kim; Jung Hyun Park; Un Chul Park; Myung Soo Cho; Haksup Lee; Seung-Yup Ku; Hyeong Gon Yu
Author Affiliations & Notes
  • Sang Jin Kim
    Department of Ophthalmology,
    Seoul National University College of Medicine, Seoul, Republic of Korea
  • Jung Hyun Park
    Department of Ophthalmology, Seoul Paik Hospital, Inje University, Seoul, Republic of Korea
  • Un Chul Park
    Department of Ophthalmology,
    Seoul National University College of Medicine, Seoul, Republic of Korea
  • Myung Soo Cho
    Research and Development Center, Jeil Pharmaceutical Co., Ltd, Seoul, Republic of Korea
    Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Republic of Korea
  • Haksup Lee
    Research and Development Center, Jeil Pharmaceutical Co., Ltd, Seoul, Republic of Korea
  • Seung-Yup Ku
    Department of Obstetrics and Gynecology,
    Seoul National University College of Medicine, Seoul, Republic of Korea
    Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Republic of Korea
  • Hyeong Gon Yu
    Department of Ophthalmology,
    Seoul National University College of Medicine, Seoul, Republic of Korea
    Institute of Reproductive Medicine and Population, Medical Research Center, Seoul National University, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  Sang Jin Kim, None; Jung Hyun Park, None; Un Chul Park, None; Myung Soo Cho, None; Haksup Lee, None; Seung-Yup Ku, None; Hyeong Gon Yu, None
  • Footnotes
    Support  This work was supported by Mid-career Researcher Program through NRF grant funded by the MEST (No. 2009-0084597).
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2227. doi:
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      Sang Jin Kim, Jung Hyun Park, Un Chul Park, Myung Soo Cho, Haksup Lee, Seung-Yup Ku, Hyeong Gon Yu; Generation of Retinal Pigment Epithelial Cells from Human Embryonic Stem Cell-derived Spherical Neural Masses. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2227.

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Abstract

Purpose: : Using spherical neural masses (SNMs), pure cell masses of embryonic stem cells derived neural precursors, is efficient and prompt method of generating functional neurons. For retinal cell replacement therapy, we regenerated functional retinal pigment epithelial (RPE) cells from human embryonic stem cells derived SNMs. We investigated morphological, molecular, and functional characteristics of RPEs from hESC-derived SNMs.

Methods: : RPEs were differentiated from human embryonic stem cells (SNUhES3) derived SNMs by neuroectodermal induction and mechanical purification. Morphologic evaluation was done with light microscopy and transmission electron microscopy. Expression of molecular markers was analyzed by RT-PCR and immunocytochemistry. After incubation of the cells with FITC-conjugated latex beads and bovine photoreceptor outer segment, confocal microscopic exam were done to evaluate phagocytosis.

Results: : Most of the differentiated cells from hESC derived SNMs showed typical RPE morphologies such as polygonal shaped epithelial monolayer with pigmentation. Transmission electron microscopic exam revealed apical microvilli, pigment granules, and tight junctions. RPE cells derived from SNMs also expressed cellular and molecular markers, Pax6, Mitf, ZO-1, RPE65, and bestrophin. Regenerated RPE function was confirmed by analysis of phagocytosis using latex bead and isolated bovine photoreceptor outer segment.

Conclusions: : RPEs were efficiently generated from hESC derived SNMs. The cells showed specific morphology, expression of molecular markers, and phagocytosis of photoreceptor outer segment.

Keywords: retinal pigment epithelium • regeneration 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2025 Association for Research in Vision and Ophthalmology.
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