Purchase this article with an account.
Peter Lundh von Leithner, Jean Lawrence, Anthony Vugler, Peter J. Coffey; Neural Progenitor Cells Differentiate Into A Glial Lineage After Intravitreal And Subretinal Transplantation In A Degenerating Retinal Environment. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2256.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To assess survival, migration and differentiation over time after early subretinal and intravitreal transplantation of neural progenitor cells in dystrophic RCS rats using in vivo confocal imaging.
GFP-labelled neural progenitor cells, derived from mouse ES cells, were transplanted into either the vitreous, or the subretinal space of immunosuppressed dystrophic RCS and age-matched control rats at P23. Cell survival and migration was gauged at successive time points using in vivo confocal fluorescence imaging. In vivo image data were subsequently correlated with histological and immunohistochemical results.
It was possible to track individual GFP-labelled cells in both intravitreally and subretinally grafted retinae in vivo. Results showed a substantial improvement of survival, and ability to migrate and integrate into host retinae of intravitreally transplanted cells compared with subretinal grafts at both 21 and 34 days post-operatively. Cell-survival and retinal integration increased in the vicinity of injury associated with transplantation in both cases. Immunohistochemical characterisation showed a predisposition in favour of glial differentiation in host RCS retinae.
The study illustrates the ability of in vivo imaging of transplanted retinae, in combination with immunohistochemical characterisation, to identify delivery routes and time points for regenerative intervention in retinal dystrophies. In this experiment neural progenitor cells successfully engrafted and migrated after both early intravitreal and subretinal transplantation into dystrophic RCS retinae. Regardless of graft placement neural progenitor cells were able to integrate and differentiate into inner retinae, albeit to a lesser extent via the subretinal route.
This PDF is available to Subscribers Only