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Hui-Rong Jiang, Heping Xu, Debbie Allan, Mei Chen, Sandra Y. Fukada, Jose C. Alves-Filho, John V. Forrester, Foo Y. Liew; IL-33 Protects Mice from Experimental Autoimmune Uveoretinitis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2278.
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IL-33 is a recently discovered IL-1 cytokine family member. Previously, we have reported that IL-33 is an important modulator of the immune system and is associated with several immune-mediated disorders. The aim of this study is to evaluate the role of IL-33 cytokine in the development of experimental autoimmune uveoretinitis (EAU).
The expression of IL-33 and its receptor ST2 on retinal pigment epithelial (RPE) cell line was examined by immunohistochemical staining. Next the severity of EAU was assessed in C57BL/6 mice treated with recombinant IL-33 or PBS. Cytokine secretion and production by the draining lymph nodes (DLNs) or spleen cells were measured at day 26 after immunization.
We demonstrate that RPE cells expressed high levels of both IL-33 and ST2. Administration of IL-33 cytokine to EAU mice led to reduced disease severity. In line with the reduced inflammation in the retina in the IL-33 treated mice, the percentage of IFN-γ+ or IL-17+ cells in the DLNs and spleen was markedly lower, while IL-5+ or IL-4+ cell percentage was increased. Furthermore, antigen specific production of IFN-γ, IL-17 and IL-6 by the DLN cells from IL-33 treated mice was also significantly reduced.
Our results suggest that IL-33 may play a protective role in the development of EAU.
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