April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
IL-33 Protects Mice from Experimental Autoimmune Uveoretinitis
Author Affiliations & Notes
  • Hui-Rong Jiang
    SIPBS, University of Strathclyde, Glasgow, United Kingdom
  • Heping Xu
    Centre for Vision and Vascular Science, Queen's University Belfast, Belfast, United Kingdom
  • Debbie Allan
    SIPBS, University of Strathclyde, Glasgow, United Kingdom
  • Mei Chen
    Centre for Vision and Vascular Science, Queen's University Belfast, Belfast, United Kingdom
  • Sandra Y. Fukada
    Immunology, Infection & Inflammation, University of Glasgow, Glasgow, United Kingdom
  • Jose C. Alves-Filho
    Immunology, Infection & Inflammation, University of Glasgow, Glasgow, United Kingdom
  • John V. Forrester
    Div of Applied Medicine, Imm & Inf, University of Aberdeen, Aberdeen, United Kingdom
  • Foo Y. Liew
    Immunology, Infection & Inflammation, University of Glasgow, Glasgow, United Kingdom
  • Footnotes
    Commercial Relationships  Hui-Rong Jiang, None; Heping Xu, None; Debbie Allan, None; Mei Chen, None; Sandra Y. Fukada, None; Jose C. Alves-Filho, None; John V. Forrester, None; Foo Y. Liew, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2278. doi:
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      Hui-Rong Jiang, Heping Xu, Debbie Allan, Mei Chen, Sandra Y. Fukada, Jose C. Alves-Filho, John V. Forrester, Foo Y. Liew; IL-33 Protects Mice from Experimental Autoimmune Uveoretinitis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2278.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : IL-33 is a recently discovered IL-1 cytokine family member. Previously, we have reported that IL-33 is an important modulator of the immune system and is associated with several immune-mediated disorders. The aim of this study is to evaluate the role of IL-33 cytokine in the development of experimental autoimmune uveoretinitis (EAU).

Methods: : The expression of IL-33 and its receptor ST2 on retinal pigment epithelial (RPE) cell line was examined by immunohistochemical staining. Next the severity of EAU was assessed in C57BL/6 mice treated with recombinant IL-33 or PBS. Cytokine secretion and production by the draining lymph nodes (DLNs) or spleen cells were measured at day 26 after immunization.

Results: : We demonstrate that RPE cells expressed high levels of both IL-33 and ST2. Administration of IL-33 cytokine to EAU mice led to reduced disease severity. In line with the reduced inflammation in the retina in the IL-33 treated mice, the percentage of IFN-γ+ or IL-17+ cells in the DLNs and spleen was markedly lower, while IL-5+ or IL-4+ cell percentage was increased. Furthermore, antigen specific production of IFN-γ, IL-17 and IL-6 by the DLN cells from IL-33 treated mice was also significantly reduced.

Conclusions: : Our results suggest that IL-33 may play a protective role in the development of EAU.

Keywords: uveitis-clinical/animal model • cytokines/chemokines • immunomodulation/immunoregulation 
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