April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Effects Of Memantine On Arpe-19 And Müller Cells (MIO-M1) Exposed To Hydroquinone (Hq) In Vitro
Claudio A. Ramirez; Khoa Pham; Marilyn Chwa; Ashish U. Sapkal; Astrid G. Limb; Baruch D. Kuppermann; M C. Kenney
Author Affiliations & Notes
  • Claudio A. Ramirez
    Gavin Herbert Eye Institute, University of California, Irvine, California
  • Khoa Pham
    Gavin Herbert Eye Institute, University of California, Irvine, Orange County, California
  • Marilyn Chwa
    Gavin Herbert Eye Institute, University of California, Irvine, Orange County, California
  • Ashish U. Sapkal
    Gavin Herbert Eye Institute, University of California, Irvine, Orange County, California
  • Astrid G. Limb
    Division of Pathology and Cell Biology, Institute of Ophtalmology, University College, London, United Kingdom
  • Baruch D. Kuppermann
    Gavin Herbert Eye Institute, University of California, Irvine, Orange County, California
  • M C. Kenney
    Gavin Herbert Eye Institute, University of California, Irvine, Orange County, California
  • Footnotes
    Commercial Relationships  Claudio A. Ramirez, None; Khoa Pham, None; Marilyn Chwa, None; Ashish U. Sapkal, None; Astrid G. Limb, None; Baruch D. Kuppermann, None; M. C. Kenney, None
  • Footnotes
    Support  Discovery Eye Foundation, Guenther Foundation, Lincy Foundation, Iris and B. Gerald Cantor Foundation, Polly and Michael Smith Foundation, Research to Prevent Blindness.
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2343. doi:
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      Claudio A. Ramirez, Khoa Pham, Marilyn Chwa, Ashish U. Sapkal, Astrid G. Limb, Baruch D. Kuppermann, M C. Kenney; Effects Of Memantine On Arpe-19 And Müller Cells (MIO-M1) Exposed To Hydroquinone (Hq) In Vitro. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2343.

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Abstract

Purpose: : To study the in vitro protective effects of memantine, a neuroptotectant used in patients with Alzheimer’s disease and glaucoma, on ARPE-19 and Müller Cells (MIO-M1) exposed to hydroquinone (HQ), one of the toxic components of cigarette smoke.

Methods: : ARPE-19 and MIO-M1 cells were pretreated for 6 hours with 30µM of memantine and then exposed to 200µM, 100µM, 50µM and 25µM HQ. Cell viability (CV) was then measured by a trypan blue dye exclusion assay and a ROS/RNS assay was performed to evaluate hydrogen peroxide, peroxyl radicals, and peroxynitrite anions.

Results: : CV for ARPE-19 cells exposed to 200µM, 100µM, 50µM and 25µM HQ was 15.40±1.27, 59.28±0.74, 74.28±1.49 and 94.85±0.86 respectively. DMSO equivalents were used as controls. Pretreatment with 30µM memantine resulted in an increase in CV at the three highest HQ doses, with values of 54.68±1.33 (p<0.0001), 90.35±1.19 (p<0.0001), 93.78±1.03 (p<0.0001) and 97.15±1.17 (p< 0.1671) respectively. CV for 200µM, 100µM, 50µM and 25µM HQ exposed MIO-M1 cells were 33.95±1.08, 38.45±1.40, 56.78±2.69 and 86.70±1.46 respectively, which increased after memantine pretreatment to 81.9±2.17 (p<0.0001), 83.83±1.17 (p<0.0001) 84.60±1.79 (p<0.0001) and 92.10±0.71 (p< 0.0163).ROS/RNS levels decreased after memantine pretreatment at the four concentrations of HQ in both cell lines. ARPE-19 cells showed fluorescence levels of 13730±759.3, 13380±622.6, 11930±367.7 and 10340±388.3 after treatment with HQ alone respectively and decreased after memantine pretreatment to 9469±876.8 (p< 0.021), 5710±938.0 (p< 0.002), 3986±838.2 (p< 0.0010) and 3566±447.2 (p< 0.0003). MIO-M1 cells treated with the same concentrations of HQ alone showed fluorescence levels of 13370±396.5, 11380±83.14, 10270±102.5 and 9977±89.63. The values decreased after memantine pretreatment to 8036±618.4 (p< 0.0019), 5043±739.5, (p< 0.0010), 3599±431.7 (p< 0.0001) and 3232±113.9 (p<0.0001) respectively.

Conclusions: : Memantine exerts an in vitro protective effect against HQ induced toxicity on human retinal pigment epithelial and Müller cells.

Keywords: age-related macular degeneration • neuroprotection • retinal culture 
© 2011, The Association for Research in Vision and Ophthalmology, Inc., all rights reserved. Permission to republish any abstract or part of an abstract in any form must be obtained in writing from the ARVO Office prior to publication.
Copyright © 2024 Association for Research in Vision and Ophthalmology.
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