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Shida Chen, Xinbo Gao, Qian Fan, Xiaolei Wang, Rui Huang, Xiulan Zhang; TRPC6 Gene Expression in Patients with Primary Open-Angle Glaucoma. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2405.
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The transient receptor potential cation channel 6 (TRPC6) is a non-selective cation channel and serves as a potential role in the survival of retinal ganglion cells. This study was to evaluate the expression level of TRPC6 gene in patients with primary open-angle glaucoma (POAG) and to validate whether the gene expression as a potential genetic biomarker for the POAG.
Peripheral blood was obtained from 84 POAG patients, 80 controls, and the total RNA was extracted from the leukocytes of peripheral blood. The real-time PCR was employed to determine the level of the TRPC6- mRNA; the related factors including age,intraocular pressure (IOP) ,the optic cup/ disk (C/D) ratio and the visual field defect were analyzed.
The result of real-time PCR showed a significant increased levels of the TRPC6-mRNA in POAG when compared to the controls (P<0.001). While the age,IOP,C/D ratio had no direct correlation with the TRPC6-mRNA level in POAG (All P>0.05). The expression level of the TRCP6 gene in the three different stages of the visual field defect had no significant difference (All P>0.05).
POAG was associated with increased TRPC6 gene expression in peripheral leukocytes that was not underlined by the factors of the age,IOP,C/D ratio and visual field status for the disease. TRPC6 gene may be considered as a significant biomarker for the POAG.
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