April 2011
Volume 52, Issue 14
ARVO Annual Meeting Abstract  |   April 2011
Gene Expression Analysis By Pcr Array After Non-penetrating Deep Sclerectomy
Author Affiliations & Notes
  • Ignacio Rodriguez-Agirretxe
    Ophthalmology, ICQO and Hospital Donostia, Bilbao, Spain
  • Iker Garcia
    R & D,
    Bioftalmik, Derio, Spain
  • Arantxa Acera
    Bioftalmik, Derio, Spain
  • Nerea Gonzalez
    Bioftalmik SL, Derio, Spain
  • Javier Soria
    Bioftalmik SL, Derio, Spain
  • Tatiana M. Suarez-Cortes
    Biomed R & D, Bioftalmik, Derio, Vizcaya, Spain
  • Footnotes
    Commercial Relationships  Ignacio Rodriguez-Agirretxe, None; Iker Garcia, Bioftalmik SL (E); Arantxa Acera, Bioftalmik (E); Nerea Gonzalez, Bioftalmik SL (E); Javier Soria, Bioftalmik SL (E); Tatiana M. Suarez-Cortes, Bioftalmik SL (E)
  • Footnotes
    Support  Ministerio de Ciencia e Innovacion, Spain (PPT-010000-2009-30)
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2425. doi:
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      Ignacio Rodriguez-Agirretxe, Iker Garcia, Arantxa Acera, Nerea Gonzalez, Javier Soria, Tatiana M. Suarez-Cortes; Gene Expression Analysis By Pcr Array After Non-penetrating Deep Sclerectomy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2425.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : To identify changes in levels of gene expression associated with wound healing and bleb failure after glaucoma surgery.

Methods: : This study was performed in 6 patients with uncontrolled open angle glaucoma (POAG) treated by nonpenetrating deep sclerectomy (NPDS). Total RNAs were isolated from conjunctiva obtained from impression cytology and peripheral blood at different moments (surgery, 15 days, 1, 3, 6 and 12 months after the NPDS). The total RNA was reversely transcribed to cDNA. We performed a RT-PCR Array based on differences between profile expressions of 88 genes previously related to wound healing. Statistical comparisons with baseline were performed with non parametric tests, correlation between IOP and gene expression was analysed with the Pearson's correlation test. Correlation between the gene expression pattern and the surgical success/failure was explored with the principal component analysis (PCA). Level of significance was P < 0.05.

Results: : When compared with baseline, 13 of 88 blood genes and 29 of 88 conjunctival genes showed expression changes. The most significant changes occurred in the conjunctiva in the first two weeks after the surgery: up-regulation of CDKN1A and 2A (4.08 fold and 5.96 fold), IL-8 (140.17 fold), TGFA (3.08 fold) and VEGFA (7.01 fold). One year after the surgery, the conjunctiva and blood recovered their baseline levels in gene expression profiles. One blood gene and 15 conjunctival genes showed an expression that correlated with IOP. The only patient with surgical failure one year after the NPDS, showed a preoperative conjunctival gene expression profile that differed from the remaining patients.

Conclusions: : Different expression patterns of mediators of the bleb wound healing process were identified. RT-PCR Array provided a powerful tool for investigating differential gene expression in wound healing in glaucoma surgery.

Keywords: gene/expression • wound healing • conjunctiva 

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