Purchase this article with an account.
Jocelyn Chua, Gemmy Cheung, Marcus Ang, Soon Phaik Chee, Jing Li, Tina T. Wong, Tina T. Wong; Expression Profile Of Inflammatory Markers In Aqueous From Glaucomatous Eyes. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2429.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
To determine if the aqueous humour from glaucomatous eyes is associated with an inflammatory profile.
Aqueous humour samples were collected from 42 eyes of 41 medically treated glaucoma (26 POAG, 12 PACG, 4 NTG eyes) patients and 23 cataract subjects during surgery in this prospective case-controlled study. A multiplexed cytokine analysis was used to quantify the presence of 20 proinflammatory cytokines in the aqueous. Data on patient demographics, duration of glaucoma, preoperative IOP as well as type and duration of anti-glaucoma therapy were collected for correlation analysis.
Mean duration of glaucoma was 53.8 months (range 1-360 months). Principal component analysis revealed separated clusters for glaucoma and cataract aqueous samples based on cytokine profiles. Overall glaucoma patient aqueous showed increased immune activities. Specifically, interleukin-8 (IL-8) (27.3±25.6 vs 12.2±16.5, p=0.01), macrophage inflammatory protein-1β (MIP-1β) (77.6±59.3 vs 43.1±40.4, p=0.01) and monokine induced by interferon-γ ((MIG) (925.9±1493.9 vs 241.1±390.1, p=0.04) levels were significantly elevated in the glaucoma group compared to cataract group. Use of carbonic anhydrase inhibitor (CAI) treatment correlated strongly with aqueous IL-4 (p=0.033, r=0.748), IL-6 (p=0.000, r=0.952), IL-7 (p=0.000, r=0.976), IL-8 (p=0.002, r=0.905), macrophage chemoattractant protein-1 (MCP-1) (p=0.002, r=0.905), INF-α2 inducible protein-10 (IP-10) (p=0.027, r=0.833), granulocyte-monocyte colony stimulating factor (GM-CSF) (p=0.043, r=0.723) and interferon-α2 (IFN-α2) (p=0.027, r=0.764).
Elevated cytokine profiles are observed in the aqueous of glaucomatous eyes and are strongly correlated with use of topical CAI.
This PDF is available to Subscribers Only