April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Expression Profile Of Inflammatory Markers In Aqueous From Glaucomatous Eyes
Author Affiliations & Notes
  • Jocelyn Chua
    Glaucoma,
    Singapore National Eye Centre, Singapore, Singapore
  • Gemmy Cheung
    Singapore National Eye Centre, Singapore, Singapore
  • Marcus Ang
    Singapore National Eye Centre, Singapore, Singapore
  • Soon Phaik Chee
    Singapore National Eye Centre, Singapore, Singapore
  • Jing Li
    Ocular Wound Healing and Therapeutics Group, Singapore Eye Research Institute, Singapore, Singapore
    Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
  • Tina T. Wong
    Glaucoma,
    Singapore National Eye Centre, Singapore, Singapore
    Ocular Wound Healing and Therapeutics Group, Singapore Eye Research Institute, Singapore, Singapore
  • Tina T. Wong
    Department of Ophthalmology, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore
    Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore
  • Footnotes
    Commercial Relationships  Jocelyn Chua, None; Gemmy Cheung, None; Marcus Ang, None; Soon Phaik Chee, None; Jing Li, None; Tina T. Wong, None; Tina T. Wong, None
  • Footnotes
    Support  NMRC Grants NMRC/EDG/0050/2009 and NMRC/TCR/002-SERI/2008
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2429. doi:
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    • Get Citation

      Jocelyn Chua, Gemmy Cheung, Marcus Ang, Soon Phaik Chee, Jing Li, Tina T. Wong, Tina T. Wong; Expression Profile Of Inflammatory Markers In Aqueous From Glaucomatous Eyes. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2429.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

To determine if the aqueous humour from glaucomatous eyes is associated with an inflammatory profile.

 
Methods:
 

Aqueous humour samples were collected from 42 eyes of 41 medically treated glaucoma (26 POAG, 12 PACG, 4 NTG eyes) patients and 23 cataract subjects during surgery in this prospective case-controlled study. A multiplexed cytokine analysis was used to quantify the presence of 20 proinflammatory cytokines in the aqueous. Data on patient demographics, duration of glaucoma, preoperative IOP as well as type and duration of anti-glaucoma therapy were collected for correlation analysis.

 
Results:
 

Mean duration of glaucoma was 53.8 months (range 1-360 months). Principal component analysis revealed separated clusters for glaucoma and cataract aqueous samples based on cytokine profiles. Overall glaucoma patient aqueous showed increased immune activities. Specifically, interleukin-8 (IL-8) (27.3±25.6 vs 12.2±16.5, p=0.01), macrophage inflammatory protein-1β (MIP-1β) (77.6±59.3 vs 43.1±40.4, p=0.01) and monokine induced by interferon-γ ((MIG) (925.9±1493.9 vs 241.1±390.1, p=0.04) levels were significantly elevated in the glaucoma group compared to cataract group. Use of carbonic anhydrase inhibitor (CAI) treatment correlated strongly with aqueous IL-4 (p=0.033, r=0.748), IL-6 (p=0.000, r=0.952), IL-7 (p=0.000, r=0.976), IL-8 (p=0.002, r=0.905), macrophage chemoattractant protein-1 (MCP-1) (p=0.002, r=0.905), INF-α2 inducible protein-10 (IP-10) (p=0.027, r=0.833), granulocyte-monocyte colony stimulating factor (GM-CSF) (p=0.043, r=0.723) and interferon-α2 (IFN-α2) (p=0.027, r=0.764).

 
Conclusions:
 

Elevated cytokine profiles are observed in the aqueous of glaucomatous eyes and are strongly correlated with use of topical CAI.

 
Keywords: wound healing • aqueous • inflammation 
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