April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
The Effects of Targeted Ablation of Melanopsin-containing Retinal Ganglion Cells on Non-image-forming Function in rd Mouse
Author Affiliations & Notes
  • Huaizhou Wang
    Beijing Tongren Eye Center, Beijing Tongren Hospital, Beijing, China
  • Jingxue Zhang
    Beijing Tongren Eye Center, Beijing Tongren Hospital, Beijing, China
  • Ningli Wang
    Beijing Tongren Eye Center, Beijing Tongren Hospital, Beijing, China
  • Footnotes
    Commercial Relationships  Huaizhou Wang, None; Jingxue Zhang, None; Ningli Wang, None
  • Footnotes
    Support  National Natural Science Foundation of China:30571991,30973261
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2450. doi:
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      Huaizhou Wang, Jingxue Zhang, Ningli Wang; The Effects of Targeted Ablation of Melanopsin-containing Retinal Ganglion Cells on Non-image-forming Function in rd Mouse. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2450.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To investigate the effects of targeted ablation of melanopsin-containing retinal ganglion cells (mcRGCs) on non-image-forming function in rd mice (C3H/HeJ).

Methods: : Different doses (100ng/ul, 200ng/ul, and 400ng/ul) of immunotoxin melanopsin-SAP were intravitreally injected into the eyes in 3 groups of rd mice respectively, the density of mcRGCs was examined on flat mount retinae. The wheel-running system was used to evaluate the non-image-forming visual function of PBS control group, 100ng/ul group and 200ng/ul group rd mice respectively.

Results: : The survival rate of mcRGCs in the 100ng/ul, 200ng/ul and 400ng/ul group was 65% ± 1.2%, 40% ± 2.8% and 40% ± 3.1% respectively. The results of free-running records system showed that: in C3H/HeJ mice, PBS control group, it took 6 days to complete the synchronization with the lighting conditions, in the 100ng/ul group, it took than 10 days to complete the synchronization with the light conditions; and in the 200ng/ul group, the mice lost the function of synchronization with the lighting conditions.

Conclusions: : Intravitreal injection of immunotoxin melanopsin-SAP can target kill the mcRGCs. In rd mice, without the function of rod and con, the loss of mcRGCs can cause the non-image-forming function change. It suggests that the intact functional mcRGCs were essential to regulate non-image-forming visual functions.

Keywords: pathology: experimental • ganglion cells • retina 
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