Purchase this article with an account.
Manuel Salinas-Navarro, Francisco Manuel Nadal-Nicolás, Leticia López-Nieto, Manuel Jiménez-López, Arturo Ortín-Martínez, Diego García-Ayuso, Caridad Galindo-Romero, Marta Agudo-Barriuso, María Paz Villegas-Pérez, Manuel Vidal-Sanz; Shortly After Ocular Hypertension There Is Loss Of Retinal Ganglion Cells, But Not Of Other Cells Of The Ganglion Cell Layer Of The Retina. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2459.
Download citation file:
© ARVO (1962-2015); The Authors (2016-present)
Laser photocoagulation (LP) of the trabecular meshwork and episcleral veins in adult rodents induces ocular hypertension (OHT) that results within four weeks in focal and sectorial loss of retinal ganglion cells (RGC) (Salinas-Navarro et al., Mol Vis 2009, 15:2578-98; Exp Eye Res. 2010, 90:168-83). Here we investigate further the mechanisms of OHT-induced RGC loss and asked whether other non-RGC cells present in the ganglion cell layer (GCL) were also affected.
In adult Sprague-Dawley rats, the intraocular pressure (IOP) of the left eye was elevated by LP of the trabecular meshwork, the limbar and episcleral veins. IOP in both eyes was measured with a Tono-Lab prior to and at 1, 7, 14 days after lasering. Animals (n=9) were analyzed at 4 weeks after LP. Retinas were prepared as whole-mounts and subjected to Brn3a immunodetection to identify surviving RGCs and to DAPI staining to detect the nuclei of all the cells laying in the GCL. Retinas were examined and photographed under fluorescence microscopy, and total numbers of Brn3a and DAPI positive nuclei in the GCL were automatically quantified.
OHT resulted in the typical focal loss of RGCs that adopted the form of wedge-like sectors with their apex on the optic nerve head devoid of Brn3a+ nuclei. These sectors were mainly localized in the dorsal retina. Within these sectors lacking RGCs there were large numbers of DAPI positive nuclei.
LP induces within four weeks a selective loss of RGCs but does not affect other cells in the GCL. It is possible that OHT induces an initial damage to RGC axons at the level of the ON head without affecting other non-RGC cells of the retina.
This PDF is available to Subscribers Only