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Elena Hernandez-Merino, Helga Kecova, Samantha J. Jacobson, Matthew M. Harper, Tatjana Lazic, Randy H. Kardon, Sinisa D. Grozdanic; Analysis Of Retinal Nerve Fiber Layer Thickness And Retinal Ganglion Cell Function During Acute Elevation Of Intraocular Pressure And Its Relationship With Ocular Perfusion Pressure In The Canine Eye. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2461.
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To determine the effect of acute episodes of elevation of intraocular pressure on nerve fiber layer (NFL) thickness and retinal ganglion cell (RGC) function and its relationship with ocular perfusion pressure. (OPP) using Spectral-Domain Optical Coherence Tomography (SD-OCT) and pattern electroretinography (pERG) in healthy canine eyes.
Acute elevation of intraocular pressure (AEIOP) was induced in 4 healthy intact male beagles. Stress testing was performed by acutely increasing intraocular pressure (IOP) to 30, 40 and 50 mmHg by anterior chamber cannulation. Optical coherence tomography (OCT) was used to evaluate the NFL thickness in the peripapillary region, area centralis region (corresponding to the human macula) and the temporal-inferior retina before and during elevation of IOP. Pattern electroretinography (pERG) and blood pressure data was collected during AEIOP to evaluate RGC function and OPP (ocular perfusion pressure). Systolic, mean and diastolic perfusion pressure (SPP, MOPP and DPP, respectively) were also evaluated.
OCT analysis of the NFL revealed a significant increase in NFL thickness in the superior quadrant of the peripapillary scan at 40 mmHg (p<0.05) and 50 mmHg (p<0.01). when compared to pre-stress testing values. The thickness of the NFL before stress testing and at IOP of 30, 40 and 50 mmHg for the inferior, temporal and nasal portions of the peripapillary scan, did not show significant change in thickness. Correlation analyses showed a significant correlation between the pERG amplitudes and the IOP (p=0.002; r2=0.3566), the MOPP (p<0.0001; r2=0.4763), the SPP (p=0.0014; r2=0.3281) and the DPP (p=0.0002; r2=0.4819). pERG amplitudes completely recovered to the pre-stress testing values.
This study revealed that an acute episode of elevated IOP produces an increase in the NFL thickness that correlates to a decrease in RGC function. We have demonstrated a significant correlation between OPP and RGC function evidenced by a decrease in pERG amplitude with a decrease in perfusion pressure.
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