March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Impact of Intravitreal Bevacizumab on Accumulation of Vascular Adhesion Protein-1 in Proliferative Diabetic Retinopathy
Author Affiliations & Notes
  • Ryo Ando
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University, Sapporo, Japan
  • Kousuke Noda
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University, Sapporo, Japan
  • Miyuki Murata
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University, Sapporo, Japan
  • Shiho Nanba
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University, Sapporo, Japan
  • Satoshi Kinoshita
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University, Sapporo, Japan
  • Junichi Fukuhara
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University, Sapporo, Japan
  • Zhenyu Dong
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University, Sapporo, Japan
  • Wataru Saito
    Department of Ophthalmology,
    Hokkaido University, Sapporo, Japan
  • Atsuhiro Kanda
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University, Sapporo, Japan
  • Susumu Ishida
    Department of Ophthalmology,
    Laboratory of Ocular Cell Biology and Visual Science,
    Hokkaido University, Sapporo, Japan
  • Footnotes
    Commercial Relationships  Ryo Ando, None; Kousuke Noda, None; Miyuki Murata, None; Shiho Nanba, None; Satoshi Kinoshita, None; Junichi Fukuhara, None; Zhenyu Dong, None; Wataru Saito, None; Atsuhiro Kanda, None; Susumu Ishida, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 2417. doi:
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      Ryo Ando, Kousuke Noda, Miyuki Murata, Shiho Nanba, Satoshi Kinoshita, Junichi Fukuhara, Zhenyu Dong, Wataru Saito, Atsuhiro Kanda, Susumu Ishida; Impact of Intravitreal Bevacizumab on Accumulation of Vascular Adhesion Protein-1 in Proliferative Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2417.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Vascular adhesion protein (VAP)-1, a multifunctional molecule with adhesive and enzymatic properties, is expressed mainly in the vascular endothelial cells of mammals. VAP-1 is known to generate hydrogen peroxide through the enzymatic activity and in turn increase the oxidative stress. The aim of this study is to investigate the effect of intravitreal bevacizumab (IVB), a humanized anti-VEGF monoclonal antibody, on the accumulation of soluble form of VAP-1 (sVAP-1) and oxidative stress in proliferative diabetic retinopathy (PDR).

Methods: : Vitreous samples were collected from 37 eyes of 37 cases with PDR (mean age, 58.8±1.4y/o), who underwent pars plana vitrectomy for prolonged vitreous hemorrhage or tractional retinal detachment involving the macular region at Hokkaido University Hospital from 2006 to 2011. This study was conducted in accordance with the tenets of the Declaration of Helsinki and after receiving approval from the institutional review committee of Hokkaido University Hospital. Of 37 eyes, 14 eyes were received the preoperative IVB (IVB group, 10 male and 4 female, 56.1±2.0y/o) and 23 eyes were not received (non-IVB group, 12 male and 11 female, 60.5±1.8y/o). Protein levels of sVAP-1 and N epsilon-(hexanoyl) lysine (HEL), a marker of oxidative stress, were measured in the vitreous samples by enzyme-linked immunosorbent assay (ELISA).

Results: : Vitreous level of sVAP-1 in IVB group was significantly lower (5.5±1.2ng/ml) compared with that in non-IVB group (9.3±1.2ng/ml, p<0.05). Similarly, the concentration of HEL in the vitreous was significantly reduced in IVB group (4.7±0.6ng/ml) than in control group (8.5±0.9ng/ml, p<0.01). Furthermore, protein concentration of sVAP-1 was positively correlated with HEL in the samples (r=0.42, p<0.01).

Conclusions: : Our data for the first time provide the evidence on the link between VEGF and sVAP-1 in eyes with PDR. The current data suggest that VEGF blockade decreases the concentration of vitreous sVAP-1 and thereby reduces the oxidative stress in PDR eyes.

Keywords: diabetic retinopathy • oxidation/oxidative or free radical damage • vitreous 
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