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Irina Semkova, Sergei Skosyrski, Michael Dutescu, Norbert Kociok, Antonia M. Joussen; Decreased Erg Response In Mice With CNV. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2445.
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To test the feasibility of recording single and multifocal electroretinograms (ERGs) from the mouse eyes with developed CNV.
ERGs were recorded 2 weeks after laser treatment of C57Bl/6 mice and their corresponding controls. Multifocal ERGs were recorded using an array of equal-sized hexagons. Local response isolation was evaluated by comparing multifocal responses to full-field ERGs and by mapping local defects in laser-treated mice. Full-field ERG with scotopic and photopic records was also performed. CNV development was identified by angiography and OCT.
Two weeks after laser treatment (5 spots per eye) CNV areas with fluorescein leakage were observed. OCT showed breakdown of the Bruch’s membrane and severe CNV lesions. Full-field ERG showed significantly decreased response in scotopic A- and B-wave. Moderate, but still significant reduction of photopic B-wave response was also observed in laser-treated mice. The most decreased response compared to the controls was the C-wave response. ERG responses were also observed by multifocal ERG.
ERG responses was significantly decreased in eyes with developed CNV. The usage of the full-field and multifocal technique will be useful in characterizing local retinal/RPE defects created with laser treatment.
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