Abstract
Purpose: :
To test the feasibility of recording single and multifocal electroretinograms (ERGs) from the mouse eyes with developed CNV.
Methods: :
ERGs were recorded 2 weeks after laser treatment of C57Bl/6 mice and their corresponding controls. Multifocal ERGs were recorded using an array of equal-sized hexagons. Local response isolation was evaluated by comparing multifocal responses to full-field ERGs and by mapping local defects in laser-treated mice. Full-field ERG with scotopic and photopic records was also performed. CNV development was identified by angiography and OCT.
Results: :
Two weeks after laser treatment (5 spots per eye) CNV areas with fluorescein leakage were observed. OCT showed breakdown of the Bruch’s membrane and severe CNV lesions. Full-field ERG showed significantly decreased response in scotopic A- and B-wave. Moderate, but still significant reduction of photopic B-wave response was also observed in laser-treated mice. The most decreased response compared to the controls was the C-wave response. ERG responses were also observed by multifocal ERG.
Conclusions: :
ERG responses was significantly decreased in eyes with developed CNV. The usage of the full-field and multifocal technique will be useful in characterizing local retinal/RPE defects created with laser treatment.
Keywords: age-related macular degeneration • retina • neovascularization