March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Abnormal Slow PIII Component Does Not Account For The Abnormal ERG b-waves Of LargeVls Mice
Author Affiliations & Notes
  • Gwen M. Sturgill-Short
    Research Service, Louis Stokes VA Medical Center, Cleveland, Ohio
  • Neal S. Peachey
    Research Service, Louis Stokes VA Medical Center, Cleveland, Ohio
    Cole Eye Institute, Cleveland Clinic Foundation, Cleveland, Ohio
  • Footnotes
    Commercial Relationships  Gwen M. Sturgill-Short, None; Neal S. Peachey, None
  • Footnotes
    Support  Department of Veterans Affairs; Foundation Fighting Blindness; Research to Prevent Blindness.
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 2459. doi:
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      Gwen M. Sturgill-Short, Neal S. Peachey; Abnormal Slow PIII Component Does Not Account For The Abnormal ERG b-waves Of LargeVls Mice. Invest. Ophthalmol. Vis. Sci. 2012;53(14):2459.

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      © ARVO (1962-2015); The Authors (2016-present)

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Purpose: : Mouse mutants for Large, fukutin and other proteins that glycosylate α-dystroglycan have abnormal ERGs in which the b-wave has a delayed onset, reduced amplitude and slow kinetics. As the b-wave is derived from the interaction of PII and slow PIII, reflecting activity of depolarizing bipolar cells (DBCs) and Müller cells, respectively, this ERG phenotype could reflect a change in the response properties of PII or slow PIII. To evaluate the possibility that the slow PIII component is abnormal in these models, we crossed the Largevls allele to a no b-wave mouse mutant background, and evaluated the response properties of slow PIII in dark-adapted ERG recordings.

Methods: : To generate study mice, we crossed the Largevls allele to a nob5 background. Mice were examined at 1 month of age. After overnight dark-adaptation, ERGs were recorded from anesthetized animals to strobe flash stimuli. ERG components generated by photoreceptors (a-wave) and Müller cells (slow PIII) were analyzed across a ~5 log unit range of stimulus luminance.

Results: : The overall waveform of Largevls/vls/nob5 mutant mice was not different from that of Largevls/+/nob5 or Large+/+/nob5 littermates. Both the a-wave and slow PIII components were reduced in amplitude in Largevls/vls/nob5 mutant mice. The magnitude of this reduction was equivalent for these two response measures.

Conclusions: : These results indicate that the abnormal b-waves noted in Largevls/vls mutant mice cannot be attributed to a change in slow PIII response properties. A similar conclusion is likely to apply to other mouse mutants with similar b-wave abnormalities.

Keywords: electroretinography: non-clinical • Muller cells • retinal degenerations: hereditary 

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