April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Intracor : Histologic And Ultrastructural Study On Human Corneas Grafts
Author Affiliations & Notes
  • Marc Doat
    Ophthalmology,
    Hotel-Dieu Hospital, Paris, France
  • Belkacem Amari
    Ophthalmology,
    Hotel-Dieu Hospital, Paris, France
    Clinique de la vision, Paris, France
  • Ossama Tazi
    Ophthalmology,
    Hotel-Dieu Hospital, Paris, France
  • Michele Savoldelli
    Ophthalmology,
    Hotel-Dieu Hospital, Paris, France
  • Jean-Louis Bourges
    Ophthalmology, Hotel-Dieu Hospital, Paris Descartes Univ, APHP, INSERM, Paris, France
  • Alain Abenhaim
    Ophthalmology,
    Hotel-Dieu Hospital, Paris, France
    Clinique de la vision, Paris, France
  • Nicolas Rocher
    Hotel-Dieu Hospital, Paris, France
  • Nicolas Fayol
    Ophthalmology,
    Hotel-Dieu Hospital, Paris, France
  • Gilles Renard
    Hotel-Dieu Hospital, Paris, France
  • Jean--Marc Legeais
    Ophthalmology,
    Hotel-Dieu Hospital, Paris, France
  • Footnotes
    Commercial Relationships  Marc Doat, None; Belkacem Amari, None; Ossama Tazi, None; Michele Savoldelli, None; Jean-Louis Bourges, None; Alain Abenhaim, None; Nicolas Rocher, None; Nicolas Fayol, None; Gilles Renard, None; Jean--Marc Legeais, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2543. doi:
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      Marc Doat, Belkacem Amari, Ossama Tazi, Michele Savoldelli, Jean-Louis Bourges, Alain Abenhaim, Nicolas Rocher, Nicolas Fayol, Gilles Renard, Jean--Marc Legeais; Intracor : Histologic And Ultrastructural Study On Human Corneas Grafts. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2543.

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Abstract

Purpose: : The Intracor represents a new treatment option of surgery for presbyopia. It consists in five concentric intrastromal cuts with the femtosecond (Technolas Perfect Vision®) without cutting through the epithelium. Its mechanism of action remains unclear. The aim of this study is to describe the intrastromal incisions histology and ultrastructure on human corneas grafts

Methods: : Our study was conducted on 10 human corneas supplied by the French Eye Bank. Histological section through the center shows corneal intrastromal incisions numbered vertical center to the periphery by the numbers 1, 2, 3, 4 and 5 at each half-cornea, called A and B. The various measurements were made on half-thin sections after staining with toluidine blue and using. For each cornea, the length of the 5 incisions, the distance between Bowman and the incision, the distance between the incision and Descemet's membrane and the total corneal thickness were measured. We calculated the average lengths of the different incisions. We also measured the distance between the incisions at 3 different levels of stromal depth.

Results: : Obtained from measurements, we calculated the average lengths (N) and standard deviations of the incisions (N1 = 299µm + / -45.72, N2 = 263µm + / -37.19, N3 = 224µm + / -32.74, N4 = 208µm + / -31.41 , N5 = 180µm + / -36.42)The diameter of the rings (A) was measured (A1 = 1690µm, 2080µm = A2, A3 = 2546µm, 2890µm = A4, A5 = 3287µm)The average spacing (E) between the incisions were measured (E1 = 187µm, 205µm = E2, E3 = 190µm, 189µm = E4, E5 = 188µm)

Conclusions: : In this study, we tried to describe for the first time, the incisions intrastromal histology and ultrastructure on human corneas grafts of corneas bank. The results show some differences between the 10 corneas linked to technical artefacts of the study. The histologic and ultrastructural study allowed us to highlight the specific architecture of intrastromal incisions on human corneas from bank Intracor laser technique. Other studies, especially in vivo, are needed to compare results and help them to consider the mode of action of this new techniqueWe compare our measurements to those referenced by Technolas,and they are rigorously close.

Keywords: cornea: basic science • refractive surgery • microscopy: electron microscopy 
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