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Beatrice E. Frueh, Markus Tschopp, Johannes Stary, Christoph Tappeiner; Voriconazole Penetration In An Ex-vivo Porcine Eye Model After Cornel Crosslinking. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2546.
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To analyze the influence of corneal crosslinking (CXL) on penetration of topically applied voriconazole.
In an ex-vivo porcine eye model, corneas were crosslinked (group 1, n=27 eyes) performing a corneal abrasion, applying riboflavin 5 mg/ml drops every 5 minutes for 30 minutes and irradiating with UVA (370 nm, 3 mW/cm2 for 30 minutes). Afterwards, eyes were rinsed with balanced salt solution (BSS). Voriconazole eye drops (10mg/ml) were topically applied onto the cornea every 5 minutes for a total of 30 minutes. In a control group (n=27 eyes) corneal abrasion and riboflavin application were performed equally, but without following UVA irradiation. Central corneal thickness and anterior chamber depth were measured using a Pentacam Scheimpflug device. In both groups, anterior chamber taps and voriconazole HPLC analysis from aqueous humor were performed.
Concentration of voriconazole in the anterior chamber was significantly lower in the eyes with previous CXL (mean 54.7±9.0 µg/ml) than in the untreated group (mean 62.9±13.0 µg/ml, p=0.01). Corneal thickness and anterior chamber volume were comparable in both groups (p>0.50, each).
CXL seems to reduce the penetration of topical applied voriconazole.
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