April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
In Vitro Biocompatibility Of Various Polymers For Retinal Prosthesis
Author Affiliations & Notes
  • Hum Chung
    Ophthalmology,
    Seoul National University Hospital, Seoul, Republic of Korea
    Nano Artificial Vision Research Center, Seoul, Republic of Korea
  • Chung Whan Che
    Clinical Research Institute,
    Seoul National University Hospital, Seoul, Republic of Korea
  • So Hyun Bae
    Ophthalmology,
    Seoul National University Hospital, Seoul, Republic of Korea
    Nano Artificial Vision Research Center, Seoul, Republic of Korea
  • Seung Woo Lee
    Nano Artificial Vision Research Center, Seoul, Republic of Korea
    Electrical Engineering, Seoul National University, Seoul, Republic of Korea
  • Kyo In Koo
    Nano Artificial Vision Research Center, Seoul, Republic of Korea
    Electrical Engineering, Seoul National University, Seoul, Republic of Korea
  • Jong-Mo Seo
    Ophthalmology,
    Seoul National University Hospital, Seoul, Republic of Korea
    Nano Artificial Vision Research Center, Seoul, Republic of Korea
  • Gregg Suaning
    Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia
  • Nigel H. Lovell
    Graduate School of Biomedical Engineering, University of New South Wales, Sydney, Australia
  • Dongil Dan Cho
    Nano Artificial Vision Research Center, Seoul, Republic of Korea
    Electrical Engineering, Seoul National University, Seoul, Republic of Korea
  • Sung June Kim
    Nano Artificial Vision Research Center, Seoul, Republic of Korea
    Electrical Engineering, Seoul National University, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  Hum Chung, None; Chung Whan Che, None; So Hyun Bae, None; Seung Woo Lee, None; Kyo In Koo, None; Jong-Mo Seo, None; Gregg Suaning, None; Nigel H. Lovell, None; Dongil Dan Cho, None; Sung June Kim, None
  • Footnotes
    Support  Korea Health 21 R & D Project A050251 by MIHWAF, Technology Innovation Program 10033634 by MKE, Happy Tech 2010-0020847 by MEST
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2588. doi:
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      Hum Chung, Chung Whan Che, So Hyun Bae, Seung Woo Lee, Kyo In Koo, Jong-Mo Seo, Gregg Suaning, Nigel H. Lovell, Dongil Dan Cho, Sung June Kim; In Vitro Biocompatibility Of Various Polymers For Retinal Prosthesis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2588.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The microelectrode array (MEA) used for retinal stimulation is arguably the most important part of a retinal prosthesis. The stability and biocompatibility of the polymer-based MEA can determine the success or failure of intraocular implantation. The purpose of this study is to evaluate the biocompatibility and cytotoxicity of several polymers used for retinal prosthesis with a standardized method.

Methods: : Three types of polymer used in MEAs were examined: silicone/platinum, polyimide/gold and liquid crystal polymer (LCP)/gold MEAs. Silicone/platinum MEAs were fabricated by EXCIMER laser (Wong YT, Vision Res. 2009;49(8):825-33), polyimide/gold MEAs by microelectromechanical systems technique (Koo KI, ARVO Abstracts 2010:51:3042), and LCP/gold MEAs by semiconductor manufacturing and thermal bonding techniques (Lee SW, IOVS 2009;50(12):5859-66). The ISO 10993-5 protocol was adopted for biocompatibility and cytotoxicity evaluation. MEAs were eluted by Minimum Essential Medium (MEM) for 24 hours and each fluid extract obtained was then applied to a cultured-cell monolayer. The morphological changes and viability of cells were analyzed by microscopic examination after 24 and 48 hr of culture. The quantification of cell viability was evaluated by MTT assay and the absorbance was measured using an ELISA plate reader.

Results: : Microscopic examination showed that none of the fluid extracts from the polymer-based MEAs affected the morphology and viability of cells after 24 and 48 hr of co-culture. The absorbance obtained through the MTT assay revealed no difference between the cell viabilities in the control well and in the wells with various diluents of each MEA.

Conclusions: : All the silicone/platinum, polyimide/gold and LCP/gold MEAs passed the ISO 10993-5 standard biocompatibility tests. Future work will examine these MEAs in chronic in vivo experiments to assess their biostability when used as a retinal prosthesis.

Keywords: retina • cell survival • visual impairment: neuro-ophthalmological disease 
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