April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Neuroprotective Effect of Resveratrol on Experimental Retinal Ischemic Injury
Author Affiliations & Notes
  • Anita P. Vin
    Ophthalmology, Research,
    Loyola University Chicago, Maywood, Illinois
  • Yougang Zhai
    Microbiology and Immunology, Neurology,
    Loyola University Chicago, Maywood, Illinois
  • Yonggang Pang
    Ophthalmology, Research,
    Biomedical Engineering, Illinois Institute of Technology, Chicago, Illinois
    Edward Hines, Jr. VA Hospital, Hines, Illinois
  • Andrew Logeman
    Stritch School of Medicine, Surgery,
    Loyola University Chicago, Maywood, Illinois
  • Hanbo Hu
    Medicine, University of Florida, Malcolm Randall VA Medical Center, Gainesville, Florida
  • Liang Qiao
    Microbiology and Immunology, Neurology,
    Loyola University Chicago, Maywood, Illinois
  • Evan B. Stubbs, Jr.
    Microbiology and Immunology, Neurology,
    Neurology,
    Loyola University Chicago, Maywood, Illinois
    Edward Hines, Jr. VA Hospital, Hines, Illinois
  • Jay Perlman
    Ophthalmology, Research,
    Stritch School of Medicine, Surgery,
    Loyola University Chicago, Maywood, Illinois
    Edward Hines, Jr. VA Hospital, Hines, Illinois
  • Ping Bu
    Ophthalmology, Research,
    Loyola University Chicago, Maywood, Illinois
    Edward Hines, Jr. VA Hospital, Hines, Illinois
  • Footnotes
    Commercial Relationships  Anita P. Vin, None; Yougang Zhai, None; Yonggang Pang, None; Andrew Logeman, None; Hanbo Hu, None; Liang Qiao, None; Evan B. Stubbs, Jr., None; Jay Perlman, None; Ping Bu, None
  • Footnotes
    Support  Illinois Society for the Prevention of Blindness; The Richard A. Perritt Charitable Foundation
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2649. doi:
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      Anita P. Vin, Yougang Zhai, Yonggang Pang, Andrew Logeman, Hanbo Hu, Liang Qiao, Evan B. Stubbs, Jr., Jay Perlman, Ping Bu; Neuroprotective Effect of Resveratrol on Experimental Retinal Ischemic Injury. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2649.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract 
 
Purpose:
 

Resveratrol has been reported to have anti-inflammatory, anti-aging, antioxidant, and anti-tumor activities. Studies have demonstrated protective effects in the spinal cord, kidneys, heart, and brain from ischemia-reperfusion injury. In this study, we investigated the neuroprotective effects of resveratrol in vitro on glutamate-induced cytotoxicity in R28 retinal precursor cells and in vivo using a pressure-induced retinal ischemic model.

 
Methods:
 

For in vitro studies, confluent cultures of R28 cells were treated with L-glutamate (0-6 mM) for 24 hours in the absence or presence of resveratrol (1-2 µg/ml). Cell viability after treatment was determined with a neutral red dye uptake assay. For in vivo studies, retinal ischemia was induced in adult male Sprague Dawley rats by intracameral elevation of intraocular pressure for 45 minutes. Following ischemic insult, rats received (intraperitoneally) an equal volume of vehicle (20% DMSO) or resveratrol (20 mg/kg) daily for 6 days. Retinal function was determined by relative changes in electroretinographic (ERG) responses from dark-adapted rats assessed prior to and 7 days after ischemic insult.

 
Results:
 

In vitro, glutamate treatment (6 mM) resulted in R28 cell death and decreased cell viability to 36±3%. The addition of resveratrol protected against glutamate-induced cytotoxicity; cell viability improved to 68±3% (1 µg/ml) and 70±6% (2 µg/ml). In vivo, prior to ischemic insult, scotopic ERG a- and b-wave amplitudes were statistically similar between the two treatment groups (average a-wave 417±51 µV and b-wave 817±90 µV). Following ischemic-reperfusion injury, ERG a- and b-wave amplitudes in vehicle-treated rats were 49±8 µV and 86±38 µV, respectively. By comparison, post-ischemia ERG a- and b-wave amplitudes in resveratrol-treated rats were 139±14 µV and 181±40 µV, respectively.

 
Conclusions:
 

Resveratrol protects cultured R28 cells against glutamate-induced cell cytotoxicity in vitro. In vivo, resveratrol-treated rats showed marked improvement in ERG a-wave and b-wave amplitudes following ischemic insult. These preliminary findings suggest that resveratrol has therapeutic value in the management of retinal ischemic diseases.

 
Keywords: ischemia • neuroprotection • drug toxicity/drug effects 
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