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Zeynep Alkin, Amir H. Kashani, Mark S. Humayun; Spectral Domain OCT Measurements of Rabbit Retinal Anatomy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2682.
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© ARVO (1962-2015); The Authors (2016-present)
To quantify the anatomical dimensions of the rabbit retina using spectral domain optical coherence tomography (SD-OCT)
Five pigmented and albino rabbits (3-4kg) were used for obtaining SD-OCT scans and fluorescein angiography with the Spectralis TM SD-OCT system. Dense raster scanning oriented perpendicular to the axis of the main vessels was performed to obtain images. Cross-sectional dimensions of retinal vessels were measured 1mm from the optic nerve head (ONH). Additional measurements obtained include retinal thickness and nerve fiber layer (NFL) thickness at 1, 2 and 3mm distances from the ONH along the medullary rays. Measurements were made on selected serial single scans of high quality with the most contrast at tissue borders (Heidelberg Viewing Module software)
All RT and NFL measurements are averages (in micrometers) from five rabbits and are reported at 1, 2, and 3mm distances from ONH respectively. Average central NFL thickness was 292 ± 4, 253 ± 9 and 211 ± 19. Peripheral NFL thickness was 130 ± 11, 98 ± 11 and 89 ± 12. Central RT was 355 ± 19, 313 ± 20 and 283 ± 18. Peripheral RT was 197 ± 15, 192 ± 19 and 179 ± 14. The main retina artery was 147 ± 10 wide and 87 ± 19 high 1mm from the ONH. The main retinal vein was 187 ± 19 wide and 107 ± 12 wide 1mm from the ONH. The figure shows a sample image with arrows indicating the measured distances. (Green = NFL, Red = Vessel Width/Height, Blue = Retinal Thickness)
We measured the cross-sectional diameters of retinal vessels, the nerve fiber layer and retinal thickness in the rabbit retina with SD-OCT. These measurements have not been reported previously and may be useful for investigators using this animal model
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