April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Investigation Of Role Of Shank 2 In Developing Mouse Retina Using Shank 2 -/- Mouse
Author Affiliations & Notes
  • Sung Wook Park
    FARB, Department of Ophthalmology, Seoul National University Hosp, Seoul, Republic of Korea
  • Jin Hyoung Kim
    FARB, Department of Ophthalmology, Seoul National University Hosp, Seoul, Republic of Korea
  • Esther Yang
    FARB, Department of Ophthalmology, Seoul National University Hosp, Seoul, Republic of Korea
  • Ku-Won Kim
    NeuroVascular Coordination Research Center, College of Pharmacy and Research Institute of Pharmaceutical Sciences, Seoul National University, Seoul, Republic of Korea
  • Young Suk Yu
    FARB, Department of Ophthalmology, Seoul National University Hosp, Seoul, Republic of Korea
  • Jeong Hun Kim
    FARB, Department of Ophthalmology, Seoul National University Hosp, Seoul, Republic of Korea
  • Footnotes
    Commercial Relationships  Sung Wook Park, None; Jin Hyoung Kim, None; Esther Yang, None; Ku-Won Kim, None; Young Suk Yu, None; Jeong Hun Kim, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2684. doi:
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      Sung Wook Park, Jin Hyoung Kim, Esther Yang, Ku-Won Kim, Young Suk Yu, Jeong Hun Kim; Investigation Of Role Of Shank 2 In Developing Mouse Retina Using Shank 2 -/- Mouse. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2684.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To elucidate the role of Shank 2 in the retinal development of Shank 2 +/+ and Shank 2 -/- mice.

Methods: : The retinae of Shank 2 +/+ and Shank 2 -/- mice at two postnatal stages, post-natal day 14 (P14) and P56, were investigated. Hematoxylin-eosin stained sections were examined for retinal pathologic changes. With immunohistochemistry(IHC) and immunofluorescence(IF), the correlation of Shank 2 and other developmental markers, such as light chain of neurofilament(NF-L), CD 31(PECAM-1) and glial fibrillary acidic protein(GFAP) were studied. The calcium/calmodulin-dependent protein kinase type II expression was also studied with IHC and IF.

Results: : Shank 2 was mainly expressed in GCL at P14, and then in GCL and inner layer of OPL at P56. In contrast to retinae from Shank2 +/+, those from Shank2 -/- exhibited a progressive loss of the IPL and disruption of ILM and OLM. In retinae from Shank 2 -/-, neurofilament expression was decreased in GCL and OPL, especially at P14. PECAM-1 expression was markedly decreased at both P14 and P56. While GFAP showed no significant difference at P 14, it was strongly expressed in GCL and IPL at P 56. CaMKII was not detected in GCL and IPL at both P14 and at P56.

Conclusions: : Our data suggest that Shank 2 was expressed in the neurons and synapses of the developing retina. The retinae of Shank 2 -/- mice might have degenerative changes in their neuronal development. Shank 2 could play a role in the neuronal differentiation and synapses of the developing retina.

Keywords: retinal development 
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