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Sung Wook Park, Jin Hyoung Kim, Esther Yang, Ku-Won Kim, Young Suk Yu, Jeong Hun Kim; Investigation Of Role Of Shank 2 In Developing Mouse Retina Using Shank 2 -/- Mouse. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2684.
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To elucidate the role of Shank 2 in the retinal development of Shank 2 +/+ and Shank 2 -/- mice.
The retinae of Shank 2 +/+ and Shank 2 -/- mice at two postnatal stages, post-natal day 14 (P14) and P56, were investigated. Hematoxylin-eosin stained sections were examined for retinal pathologic changes. With immunohistochemistry(IHC) and immunofluorescence(IF), the correlation of Shank 2 and other developmental markers, such as light chain of neurofilament(NF-L), CD 31(PECAM-1) and glial fibrillary acidic protein(GFAP) were studied. The calcium/calmodulin-dependent protein kinase type II expression was also studied with IHC and IF.
Shank 2 was mainly expressed in GCL at P14, and then in GCL and inner layer of OPL at P56. In contrast to retinae from Shank2 +/+, those from Shank2 -/- exhibited a progressive loss of the IPL and disruption of ILM and OLM. In retinae from Shank 2 -/-, neurofilament expression was decreased in GCL and OPL, especially at P14. PECAM-1 expression was markedly decreased at both P14 and P56. While GFAP showed no significant difference at P 14, it was strongly expressed in GCL and IPL at P 56. CaMKII was not detected in GCL and IPL at both P14 and at P56.
Our data suggest that Shank 2 was expressed in the neurons and synapses of the developing retina. The retinae of Shank 2 -/- mice might have degenerative changes in their neuronal development. Shank 2 could play a role in the neuronal differentiation and synapses of the developing retina.
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