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Shuming Sun, Long Liu, Xiaoming Zeng, Lan Zhang, Jiao Liu, Pei-Qiao Chen, Wen-Feng Hu, Xiao-Hui Hu, David W. Li; The Lens Stem Cells Appear To Be Localized In The Central Epithelium Of The Ocular Lens. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2787.
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The ocular lens maintains life-long growth. Previous studies have shown that the lens cells in the germinal zone are responsible for generating the secondary lens fiber cells. However, it remains to be determined where the lens stem cells are localized.
Telomerase activity was assayed in both central and periphery zones of young rabbit lens epithelium. Antibodies against a panel of 8 stem cell markers were used to conduct both immunocytochemistry and western blot analysis. In vitro culture and induction of cell differentiation of lens epithelial cells from both central and periphery zones were conducted.
The central epithelial cells display telomerase-positive activity. Several stem cell markers are expressed in the central epithelium of the ocular lens. The central zone of lens epithelial cells can be induced to undergo differentiation.
The lens stem cells appear to be localized in the central epithelium of the ocular lens.
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