April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
The Lens Stem Cells Appear To Be Localized In The Central Epithelium Of The Ocular Lens
Author Affiliations & Notes
  • Shuming Sun
    Key Lab. of Protein Chemistry & Developmental Biology, Hunan Normal University, Changsha, Hunan 410081, China
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Long Liu
    Key Lab. of Protein Chemistry & Developmental Biology, Hunan Normal University, Changsha, Hunan 410081, China
  • Xiaoming Zeng
    Key Lab. of Protein Chemistry & Developmental Biology, Hunan Normal University, Changsha, Hunan 410081, China
  • Lan Zhang
    Key Lab. of Protein Chemistry & Developmental Biology, Hunan Normal University, Changsha, Hunan 410081, China
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Jiao Liu
    Key Lab. of Protein Chemistry & Developmental Biology, Hunan Normal University, Changsha, Hunan 410081, China
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Pei-Qiao Chen
    Key Lab. of Protein Chemistry & Developmental Biology, Hunan Normal University, Changsha, Hunan 410081, China
  • Wen-Feng Hu
    Key Lab. of Protein Chemistry & Developmental Biology, Hunan Normal University, Changsha, Hunan 410081, China
  • Xiao-Hui Hu
    Key Lab. of Protein Chemistry & Developmental Biology, Hunan Normal University, Changsha, Hunan 410081, China
  • David W. Li
    Key Lab. of Protein Chemistry & Developmental Biology, Hunan Normal University, Changsha, Hunan 410081, China
    Biochemistry & Molecular Biology, University of Nebraska Medical Center, Omaha, Nebraska
  • Footnotes
    Commercial Relationships  Shuming Sun, None; Long Liu, None; Xiaoming Zeng, None; Lan Zhang, None; Jiao Liu, None; Pei-Qiao Chen, None; Wen-Feng Hu, None; Xiao-Hui Hu, None; David W. Li, None
  • Footnotes
    Support  EY018380
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2787. doi:
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      Shuming Sun, Long Liu, Xiaoming Zeng, Lan Zhang, Jiao Liu, Pei-Qiao Chen, Wen-Feng Hu, Xiao-Hui Hu, David W. Li; The Lens Stem Cells Appear To Be Localized In The Central Epithelium Of The Ocular Lens. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2787.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : The ocular lens maintains life-long growth. Previous studies have shown that the lens cells in the germinal zone are responsible for generating the secondary lens fiber cells. However, it remains to be determined where the lens stem cells are localized.

Methods: : Telomerase activity was assayed in both central and periphery zones of young rabbit lens epithelium. Antibodies against a panel of 8 stem cell markers were used to conduct both immunocytochemistry and western blot analysis. In vitro culture and induction of cell differentiation of lens epithelial cells from both central and periphery zones were conducted.

Results: : The central epithelial cells display telomerase-positive activity. Several stem cell markers are expressed in the central epithelium of the ocular lens. The central zone of lens epithelial cells can be induced to undergo differentiation.

Conclusions: : The lens stem cells appear to be localized in the central epithelium of the ocular lens.

Keywords: aging • signal transduction • gene/expression 
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