April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
The Expression Of Pd-l1 And Pd-l2 On Human Retinal Pigment Epithelial Cells Are Differentially Regulated By Cytokines
Author Affiliations & Notes
  • Jia Ni
    Laboratory of Immunology, NEI, Bethesda, Maryland
  • Lai Wei
    Laboratory of Immunology, NEI, Bethesda, Maryland
  • Baoying Liu
    Laboratory of Immunology, NEI, Bethesda, Maryland
  • Zhiyu Li
    Laboratory of Immunology, NEI, Bethesda, Maryland
  • Chandrasekharam N. Nagineni
    Laboratory of Immunology, NEI, Bethesda, Maryland
  • Robert B. Nussenblatt
    Laboratory of Immunology, NEI, Bethesda, Maryland
  • Footnotes
    Commercial Relationships  Jia Ni, None; Lai Wei, None; Baoying Liu, None; Zhiyu Li, None; Chandrasekharam N. Nagineni, None; Robert B. Nussenblatt, None
  • Footnotes
    Support  NIH Intramural research programme
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 2921. doi:
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      Jia Ni, Lai Wei, Baoying Liu, Zhiyu Li, Chandrasekharam N. Nagineni, Robert B. Nussenblatt; The Expression Of Pd-l1 And Pd-l2 On Human Retinal Pigment Epithelial Cells Are Differentially Regulated By Cytokines. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2921.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Recent studies have shown that human retinal pigment epithelial (RPE) cells have the ability to suppress T-cell activation, and the suppressor function may relate to the expression of PD-L1 and PD-L2. The goal of this study is to investigate the expression of PD-L1 and PD-L2 regulation by different cytokines.

Methods: : Human retinal pigment epithelial (HRPE) cells from a 89 years old donor were cultured with various cytokines for 48 hours. The cells were collected and stained with anti-PD-L1 and PD-L2 antibodies. The expression of PD-L1 and PD-L2 were analyzed by a FACS Caliber flow cytometer.

Results: : HRPE cells constitutively expressed a low level of PD-L1 and PD-L2, and after exposure for 48 h to IFN-γ, both the PD-L1 and PD-L2 expression were markedly increased. Exposure for 48 h to the Th2 cytokines IL-4 and IL-13, but not IL-5, could up-regulate the expression of PD-L2 as well. The Th2 cytokines IL-4, IL-13 and IL-5, had no effect on the expression of PD-L1. Moreover, we found that IFN-γ had a synergistic effect with IL-4 or IL-13 on the expression of PD-L2. The exposure to other pro-inflammatory cytokines including, IL-1, IL-6, TNF-α, IL-17 did not affect the expression of PD-L1 and PD-L2 on HRPE cells.

Conclusions: : PD-L1 and PD-L2 can inhibit T cell activation, proliferation and cytokine production. Our studies showed the expression of PD-L1 and PD-L2 could be regulated by different cytokines. PD-L1 and PD-L2 expression by HRPE cells on exposure to different cytokines might promote local anti-inflammatory responses and accelerate the resolution of uveitis.

Keywords: retinitis • immunomodulation/immunoregulation • inhibitory receptors 
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