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Delu Song, Renyan Wang, Yong Zhong, Fangtian Dong; Aberrant Secretion Of Insulin-like Growth Factor-1 By Orbital Fibroblasts From Patients With Thyroid Associated Ophthalmopathy In Vitro: Implications For Disease Pathogenesis. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2940.
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To investigate the secretion effect of Insulin-like Growth Factor-1 (IGF-1) by orbital fibroblasts from patients with Thyroid Associated Ophthalmopathy (TAO) and to determine whether serum levels of total and free IGF-1 are normal and its relationship with clinical activity scores.
Eighteen consecutive patients with TAO, sixteen age and sex matched controls were studied. Total and free IGF-1 in serum were determined using a noncompetitive time resolved radioimmunoassy kit. The IGF-1 concentration in cell cultural media was determined by Elisa kit.
Total and free IGF-1 levels in patients were similar to controls and did not show any significant difference (Total IGF-1: TAO patients=121±52 Control=149±45, Free IGF-1: TAO patients=0.54±0.19 Control=0.79±0.32). Furthermore, no statistically significant differences emerged in above mentioned parameters and clinical activity scores. Radioimmunoassay demonstrated that orbital fibroblasts from both TAO patients and normal controls produce IGF-1 and this production continued over a 72 hour period. IGF-1 production in orbital fibroblasts from TAO patients is significant more than normal controls.
No relationship was found between the IGF-1 levels in serum of TAO patients with clinical activity scores. Autocrine of IGF-1 from orbital fibroblast is over activated in TAO patients and may play a significant role in pathogenesis of TAO.
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