Abstract
Purpose: :
To investigate the secretion effect of Insulin-like Growth Factor-1 (IGF-1) by orbital fibroblasts from patients with Thyroid Associated Ophthalmopathy (TAO) and to determine whether serum levels of total and free IGF-1 are normal and its relationship with clinical activity scores.
Methods: :
Eighteen consecutive patients with TAO, sixteen age and sex matched controls were studied. Total and free IGF-1 in serum were determined using a noncompetitive time resolved radioimmunoassy kit. The IGF-1 concentration in cell cultural media was determined by Elisa kit.
Results: :
Total and free IGF-1 levels in patients were similar to controls and did not show any significant difference (Total IGF-1: TAO patients=121±52 Control=149±45, Free IGF-1: TAO patients=0.54±0.19 Control=0.79±0.32). Furthermore, no statistically significant differences emerged in above mentioned parameters and clinical activity scores. Radioimmunoassay demonstrated that orbital fibroblasts from both TAO patients and normal controls produce IGF-1 and this production continued over a 72 hour period. IGF-1 production in orbital fibroblasts from TAO patients is significant more than normal controls.
Conclusions: :
No relationship was found between the IGF-1 levels in serum of TAO patients with clinical activity scores. Autocrine of IGF-1 from orbital fibroblast is over activated in TAO patients and may play a significant role in pathogenesis of TAO.
Clinical Trial: :
www.pumchkyc.net, 20080502012
Keywords: autoimmune disease