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Alicia R. Perry, Philip Huie, Luo Luo Zheng, Tatyana Milman, Christopher N. Ta; Using India Ink to Determine Bacterial Infiltration of Intravitreal Injections. Invest. Ophthalmol. Vis. Sci. 2011;52(14):2956.
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To determine the possibility of bacterial infiltration of wounds created by a 30 gauge intravitreal injection needle.
Sclera was excised from whole fresh pig eyes. A 30 gauge needle was used to perforate the sclera, which was then placed into an osmotically neutral diffusion chamber with balanced salt solution (BSS) on one side and 10% India ink in BSS on the other. After 3 days, the sclera was removed from the diffusion chambers, cleaned, and fixed in 10% formalin. Histology was performed using hematoxylin and eosin stain.
Histopathologic evaluation revealed that the India ink particles were visible throughout all wounds.
India ink particles can infiltrate full thickness scleral wounds created by a 30 gauge needle in the absence of intraocular pressure. Since India ink particles and bacteria are of a similar size, our results indicate that bacteria have the potential to infiltrate the wound after an intravitreal injection, suggesting the need for post-injection antibiotics.
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