Proteomics In Exudative Amd

Abstract

 

Purpose:

 

To analyze and compare vitreous samples of exudative AMD with these of floater patients with PROTEOMICS.

 

Methods:

 

Twenty-three vitreous samples (15 AMD and 8 controls) from patients undergoing 23 gauge single-site core vitrectomy for combined intravitreal treatment were analyzed with capillary electrophoresis coupled to mass spectrometry.

 

Results:

 

A total of 5949 polypeptides (PP) could be defined in all samples based on mass, migration time, and normed amplitude. A mean of 167±149PP were annotated in the controls (C), 380±178PP in the AMD group (p=0.0098). Out of 43PP (figure 1), which were significantly different between the groups, several could be identified by their amino acid sequence.

 

Conclusions:

 

The number of annotated and detected PP between AMD and controls was significantly different. Specific biomarkers that are significantly associated with AMD could be identified, among those are Fibrinogen alpha chain, Calsyntenin-1, Amyloid-like protein 2, and Osteopontin.  

 

Keywords: age-related macular degeneration • proteomics