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Shuichiro Hirahara, Takeshi Mizutani, Aiko Ito, Miho Nozaki, Yuichiro Ogura; Pattern Scan Laser Photocoagulation induces less VEGF expression than conventional laser in the murine retina. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3023.
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© ARVO (1962-2015); The Authors (2016-present)
The pattern scan laser is a semiautomated photocoagulator that delivers a pattern array of multiple burns in a rapid predetermined sequence. Histological evaluation revealed that the pattern scan laser system might restore inner retina. Recently, not only green wavelength (532 nm) but also yellow wavelength (577 nm) became available with the pattern scan laser system. Previously, we have reported that the pattern scan laser with green wavelength induced less inflammatory cytokine including VEGF in the sensory retina compare with conventional laser treatment (Hirano Y, et al, ARVO, 2010 and Sakamoto M, et al, ARVO, 2011). The purpose of this study is to compare the expression pattern of VEGF in the murine retina between the patter scan laser with green or yellow wavelength.
Retinal scatter laser photocoagulation was performed on C57BL/6J mice using the pattern scan laser with green wavelength (532 nm), yellow wavelength (577 nm) (PASCAL Streamline,Topcon, Tokyo) or a conventional laser (532nm) (Novus Varia®,Lumenis, CA). The eyes were enucleated 1 and 3 days after laser treatment. The levels of VEGF in the sensory retina and RPE-choroid were quantified by ELISA. Immunohistochemistry was also performed for VEGF and macrophage infiltration (F4/80) on day 3.
VEGF was significantly elevated in the sensory retina after laser treatment in conventional laser group on day 1 (P<0.05). In pattern scan laser group, both 532 nm and 577 nm induced VEGF expression, but there was no significant difference compared with the level on day 0.
We have shown that pattern scan laser induced less VEGF in the sensory retina compare with conventional laser treatment. And there was no significant difference between green and yellow wavelength. Collectively, the pattern scan laser system, short duration and high power settings, may affect less on inner retina, and led to support safety of panretinal photocoagulation with onesession.
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