March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Clinical Study of alpha crystallin, the lens molecular chaperone, in the various lens compartments in normal subjects and cataract patients using Dynamic Light Scattering
Author Affiliations & Notes
  • Qing Pan
    Wilmer Eye Institute,Johns Hopkins University, Baltimore, Maryland
  • Li Tang
    Wilmer Eye Institute,Johns Hopkins University, Baltimore, Maryland
  • Manuel B. Datiles, III
    National Eye Institute-NIH, Bethesda, Maryland
  • Rafat Ansari
    NASA-Glenn Research Center, Cleveland, Ohio
  • J.Samuel Zigler Jr.
    Wilmer Eye Institute,Johns Hopkins University, Baltimore, Maryland
  • James F. King
    NASA-Glenn Research Center, Cleveland, Ohio
  • Junko Yoshida
    Wilmer Eye Institute,Johns Hopkins University, Baltimore, Maryland
  • Frederick Ferris
    National Eye Institute-NIH, Bethesda, Maryland
  • Jing Tian
    Wilmer Eye Institute,Johns Hopkins University, Baltimore, Maryland
  • Walter J. Stark
    Wilmer Eye Institute,Johns Hopkins University, Baltimore, Maryland
  • Footnotes
    Commercial Relationships  Qing Pan, None; Li Tang, None; Manuel B. Datiles, III, None; Rafat Ansari, Patent No. 5973779 (P); J.Samuel Zigler Jr., None; James F. King, None; Junko Yoshida, None; Frederick Ferris, None; Jing Tian, None; Walter J. Stark, None
  • Footnotes
    Support  NEI Intramural Research Program
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3059. doi:
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      Qing Pan, Li Tang, Manuel B. Datiles, III, Rafat Ansari, J.Samuel Zigler Jr., James F. King, Junko Yoshida, Frederick Ferris, Jing Tian, Walter J. Stark; Clinical Study of alpha crystallin, the lens molecular chaperone, in the various lens compartments in normal subjects and cataract patients using Dynamic Light Scattering. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3059.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To clinically estimate the levels of the molecular chaperone , α-crystallin, in different regions of the human lens in vivo using the NASA-NEI Dynamic Light Scattering (DLS) device.

Methods: : A clinic-based cohort of 112 persons from 20 to 90 years of age (Mean±SD: 54.63±13.32 years) were included in this approved study by NEI-IRB. The gender ratio (Female /Male) was 51/61. All tenets of the Declaration of Helsinki were followed and each participant gave their informed consent. The NEI-NASA DLS device was used to measure α-crystallin index (ACI) in vivo at nuclear, anterior and posterior cortical areas in participants’ lenses.Participants were examined by slit-lamp and lenses were graded for nuclear, cortical, and posterior subcapsular opacities using the AREDS system for classifying cataracts.

Results: : DLS assessment in normal clear lenses revealed that ACI was significantly abundant at lens nucleus (16.17±6.20%) and anterior cortex (15.67±9.03%), compared to posterior cortex (12.44±7.35%, both p<0.05). There was a significant inverse correlation between age and DLS -ACI at all three regions in noncataractous lens: specifically, ACI significantly decreased 0.40±0.05%, 0.30±0.03% and 0.18 ± 0.07% per year at nucleus, anterior cortex, and posterior cortex respectively (all, p<0.05, Unvaried analysis). However, gender was not shown to be correlated with DLS-ACI at any of the three regions in normal lenses in this cohort study (age-adjusted multivariate analysis). In addition, DLS-ACI at nucleus and anterior cortical lens were found significantly to be associated inversely with nuclear lens opacity (0~3.8; p<0.001) and cortical opacity grading (0~3.5; p<0.05) respectively. However, ACI at posterior cortical lens was not associated with cortical opacity grading (0~3.5; p=0.43).

Conclusions: : The anti cataract protein α-crystallin declines with age in human nuclear, anterior and posterior cortical lens. A noninvasive DLS device could clinically track the level of lens α-crystallins and predict impending nuclear and cortical cataract formation.

Keywords: crystallins • aging • cataract 
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