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Qing Pan, Li Tang, Manuel B. Datiles, III, Rafat Ansari, J.Samuel Zigler Jr., James F. King, Junko Yoshida, Frederick Ferris, Jing Tian, Walter J. Stark; Clinical Study of alpha crystallin, the lens molecular chaperone, in the various lens compartments in normal subjects and cataract patients using Dynamic Light Scattering. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3059.
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© ARVO (1962-2015); The Authors (2016-present)
To clinically estimate the levels of the molecular chaperone , α-crystallin, in different regions of the human lens in vivo using the NASA-NEI Dynamic Light Scattering (DLS) device.
A clinic-based cohort of 112 persons from 20 to 90 years of age (Mean±SD: 54.63±13.32 years) were included in this approved study by NEI-IRB. The gender ratio (Female /Male) was 51/61. All tenets of the Declaration of Helsinki were followed and each participant gave their informed consent. The NEI-NASA DLS device was used to measure α-crystallin index (ACI) in vivo at nuclear, anterior and posterior cortical areas in participants’ lenses.Participants were examined by slit-lamp and lenses were graded for nuclear, cortical, and posterior subcapsular opacities using the AREDS system for classifying cataracts.
DLS assessment in normal clear lenses revealed that ACI was significantly abundant at lens nucleus (16.17±6.20%) and anterior cortex (15.67±9.03%), compared to posterior cortex (12.44±7.35%, both p<0.05). There was a significant inverse correlation between age and DLS -ACI at all three regions in noncataractous lens: specifically, ACI significantly decreased 0.40±0.05%, 0.30±0.03% and 0.18 ± 0.07% per year at nucleus, anterior cortex, and posterior cortex respectively (all, p<0.05, Unvaried analysis). However, gender was not shown to be correlated with DLS-ACI at any of the three regions in normal lenses in this cohort study (age-adjusted multivariate analysis). In addition, DLS-ACI at nucleus and anterior cortical lens were found significantly to be associated inversely with nuclear lens opacity (0~3.8; p<0.001) and cortical opacity grading (0~3.5; p<0.05) respectively. However, ACI at posterior cortical lens was not associated with cortical opacity grading (0~3.5; p=0.43).
The anti cataract protein α-crystallin declines with age in human nuclear, anterior and posterior cortical lens. A noninvasive DLS device could clinically track the level of lens α-crystallins and predict impending nuclear and cortical cataract formation.
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