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Kostadinka K. Bizheva, Chulho Hyun, Azhar Zam, Chenyi Liu, Abhishek Kumar, Alexander Wong, Natalie Hutchings, Trefford Simpson; In vivo Volumetric Imaging of the Vogt Palisades with Ultrahigh Resolution Optical Coherence Tomography. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3078.
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© ARVO (1962-2015); The Authors (2016-present)
To image the human Vogt palisades in vivo at cellular level resolution and in 3D.
A research-grade UHR-OCT system was developed for in vivo volumetric, cellular level resolution imaging of the anterior chamber of the human eye. The system operates at ~800nm and provides 1.4µm axial and 4µm lateral resolution at the rate of 70,000 A-scans per second. Volumetric UHR-OCT tomograms of the limbus were obtained from 10 healthy subjects. Novel image processing algorithms were developed for flattening and 3D rendering of the UHR-OCT tomogram to allow for better visualization of morphological features located at various depths, independently from the tissue surface curvature. Cross-sectional UHR-OCT tomograms of the human limbus were compared with textbook histology of healthy limbus and high resolution en-face confocal images.
Cross-sectional and volumetric images of the healthy limbus revealed in great detail the highly reflective trabecular extensions of the Vogt palisades, as well as areas in between of lower reflectivity, that are considered populated with stem cells. The volumetric OCT tomograms also revealed a network of micro-channels that appear to connect the Vogt palisades with the cornea. An intricate network of blood and lymph vessels was visualized in the volumetric scans directly below the Vogt palisades. A representative cross-sectional OCT image of the corneo-scleral limbus is shown below with read arrows marking the trabecular extensions and the white arrows - the underlying vasculature.
We demonstrated the ability of novel, research grade UHR-OCT to provide in-vivo volumetric cellular level visualization of fine morphological details in the corneo-scleral human limbus. This technology has great potential for diagnosing and monitoring the clinical course of limbal stem cell deficiency and possibly limbal cell activity.
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