April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Prednisolone Acetate Loaded Biodegradable Microfilm Attenuates The Post-operative Inflammatory Response Following Experimental Glaucoma Filtration Surgery
Author Affiliations & Notes
  • Marcus Ang
    Singapore National Eye Center, Singapore, Singapore
    Singapore Eye Research Institute, Singapore, Singapore
  • Peng Yan
    Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore
  • Ma Zhen
    Singapore Eye Research Institute, Singapore, Singapore
    Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore
  • Selin Foo
    Singapore Eye Research Institute, Singapore, Singapore
  • Wing Sum Lee
    Singapore Eye Research Institute, Singapore, Singapore
  • Subbu S. Venkatraman
    Materials Science and Engineering, Nanyang Technological University, Singapore, Singapore
  • Tina T. Wong
    Singapore Eye Research Institute, Singapore, Singapore
    Glaucoma, Singapore National Eye Centre, Singapore, Singapore
  • Footnotes
    Commercial Relationships  Marcus Ang, None; Peng Yan, None; Ma Zhen, None; Selin Foo, None; Wing Sum Lee, None; Subbu S. Venkatraman, None; Tina T. Wong, None
  • Footnotes
    Support  National Research Foundation-Funded Translational & Clinical Research (TCR) Programme Grant [R619/40/2008/TCR]
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3221. doi:
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      Marcus Ang, Peng Yan, Ma Zhen, Selin Foo, Wing Sum Lee, Subbu S. Venkatraman, Tina T. Wong; Prednisolone Acetate Loaded Biodegradable Microfilm Attenuates The Post-operative Inflammatory Response Following Experimental Glaucoma Filtration Surgery. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3221.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : To evaluate the efficacy of a biodegradable microfilm providing sustained release of prednisolone acetate on modulating post-operative inflammation and improving bleb survival after experimental glaucoma filtration surgery.

Methods: : We fabricated biodegrable microfilms composed of poly (d, l-lactide-co-caprolactone) (PLC) 70/30 which were pre-loaded with 20% total weight of prednisolone actetate. We randomised 14 female New Zealand White rabbits into 3 treatment groups: filtration surgery alone (n=4), filtration surgery with PLC microfilms (n=4) and filtration surgery with prednisolone acetate loaded PLC microfilm (n=6). Microfilms were inserted in the subconjunctival pocket adjacent to the site of filtration. We clinically monitored the rabbits with slit-lamp microscopy; bleb photography and anterior segment optical coherence tomography (ASOCT). Animals were sacrificed after 28 days and histology performed to identify any signs of inflammation or subconjunctival scarring.

Results: : Our in vivo study results revealed a steady, linear, sustained release of prednisolone acetate from the 20% prednisolone-loaded microfilms. Eyes implanted with prednisolone-loaded PLC microfilms demonstrated significantly better bleb survival and reduced bleb conjunctival vascularity, as compared to the other two treatment groups. All eyes implanted with prednisolone-loaded microfilms (n=6) had blebs that survived at 30 days, while control eyes with no microfilms (n=4) and control eyes with blank microfilms (n=4) had all failed by median 17.5 (range, 14 -21) days. On ASOCT, significant blebs were visible in all eyes (6 eyes) with prednisolone-loaded microfilms as compared to the other treatment groups, where only 3/8 eyes (37.5%) had visible blebs (P<0.001). Our observations were supported by the presence of significantly less subconjunctival inflammation and scarring around the filtration site in the prednisolone-loaded PLC microfilm treated group on histology.

Conclusions: : Sustained release of prednisolone acetate from biodegradable microfilms can successfully reduce the post-surgical inflammatory response and thereby prolong bleb survival in this rabbit surgical model.

Keywords: optic disc • outflow: trabecular meshwork 
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