April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Patterns Of Light-mediated C-fos Activity In The Medial Visual Cortex Of Mice Lacking Both Rods And Cones
Author Affiliations & Notes
  • F Javier Valiente-Soriano
    Ophthalmology, Universidad de Murcia, Murcia, Spain
  • Diego García-Ayuso
    Ophthalmology, Universidad de Murcia, Murcia, Spain
  • Arturo Ortín-Martínez
    Ophthalmology, Universidad de Murcia, Murcia, Spain
  • Ma'ayan Semo
    ORBIT, UCL-Institute of Ophthalmology, London EC1V 9EL, United Kingdom
  • Manuel Jiménez-López
    Ophthalmology, Universidad de Murcia, Murcia, Spain
  • Carlos Gias
    ORBIT, UCL-Institute of Ophthalmology, London EC1V 9EL, United Kingdom
  • Maria Paz Villegas-Pérez
    Ophthalmology, Universidad de Murcia, Murcia, Spain
  • Peter Coffey
    ORBIT, UCL-Institute of Ophthalmology, London EC1V 9EL, United Kingdom
  • Manuel Vidal-Sanz
    Ophthalmology, Universidad de Murcia, Murcia, Spain
  • Anthony A. Vugler
    ORBIT, UCL-Institute of Ophthalmology, London EC1V 9EL, United Kingdom
  • Footnotes
    Commercial Relationships  F Javier Valiente-Soriano, None; Diego García-Ayuso, None; Arturo Ortín-Martínez, None; Ma'ayan Semo, None; Manuel Jiménez-López, None; Carlos Gias, None; Maria Paz Villegas-Pérez, None; Peter Coffey, None; Manuel Vidal-Sanz, None; Anthony A. Vugler, None
  • Footnotes
    Support  Fundación Séneca 04446/GERM/07; Spanish Ministry of Education and Science SAF 2009-10385; ISCIII-FEDER FIS PIO06/0780, PI 10/01496; RD07/0062/0001; The London Project to Cure Blindness
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3464. doi:
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      F Javier Valiente-Soriano, Diego García-Ayuso, Arturo Ortín-Martínez, Ma'ayan Semo, Manuel Jiménez-López, Carlos Gias, Maria Paz Villegas-Pérez, Peter Coffey, Manuel Vidal-Sanz, Anthony A. Vugler; Patterns Of Light-mediated C-fos Activity In The Medial Visual Cortex Of Mice Lacking Both Rods And Cones. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3464.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract
 
Purpose:
 

Retinal melanopsin cells are capable of activating the visual cortex in mice (Brown et al., 2010; Ecker et al., 2010). In order to investigate the anatomical extent of this response we have examined light-driven c-fos expression in the medial visual cortex of mice lacking rods/cones (rd/rd cl).

 
Methods:
 

Mice were dark-adapted overnight and either sacrificed in darkness or following 90 minutes light (250µW/cm2). Coronal sections through the brain were immunostained for c-fos together with the cytoarchitectural markers SMI-32, calretinin, calbindin, parvalbumin and GAD67. Light-induced neural activation was measured across the rostro-caudal extent of visual cortex by quantification of c-fos positive cells. In both the dark-adapted and light-exposed groups, analysis was restricted to a region of interest in medial V1 / V2.

 
Results:
 

There was a marked elevation in c-fos positive cells in the visual cortex of rd/rd cl mice following light exposure. This effect achieved statistical significance towards more caudal regions of medial visual cortex (p<0.05). While light-driven cortical activation was observed throughout all layers of visual cortex (I-VI), there was a marked response in layer IV (input) and the deepest layer (VI). The c-fos positive cells were associated with immunoreactivity for GAD67 and a small subpopulation expressed the inhibitory interneuron marker calbindin.

 
Conclusions:
 

Using the functional anatomical marker c-fos, we provide evidence that the melanopsin system is capable of activating more middle-caudal regions of visual cortex in retinal degenerate mice. A subset of activated neurons express markers consistent with inhibitory neurotransmission and the activation in layer VI may suggest a role for melanopsin in the modulation of dLGN sensitivity / visual object recognition.  

 
Keywords: ganglion cells • visual cortex • retina 
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