April 2011
Volume 52, Issue 14
Free
ARVO Annual Meeting Abstract  |   April 2011
Robust Diabetes-induced Retinal Thickening in Male Sprague Dawley Rats In Vivo: Inhibition by Angiotensin Converting Enzyme Inhibition (ACEi) Treatment
Author Affiliations & Notes
  • Bruce A. Berkowitz
    Anatomy/Cell Biol & Ophthal,
    Wayne State Univ Sch of Med, Detroit, Michigan
  • David Bissig
    Anatomy/Cell Biol,
    Wayne State Univ Sch of Med, Detroit, Michigan
  • Timothy S. Kern
    Department of Medicine, Case Western Reserve Univ, Cleveland, Ohio
  • Robin Roberts
    Anatomy & Cell Biol,
    Wayne State Univ Sch of Med, Detroit, Michigan
  • Footnotes
    Commercial Relationships  Bruce A. Berkowitz, None; David Bissig, None; Timothy S. Kern, None; Robin Roberts, None
  • Footnotes
    Support  JDRF (BAB), NIH EY00300 (TSK), Research to Prevent Blindness (BAB)
Investigative Ophthalmology & Visual Science April 2011, Vol.52, 3555. doi:https://doi.org/
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      Bruce A. Berkowitz, David Bissig, Timothy S. Kern, Robin Roberts; Robust Diabetes-induced Retinal Thickening in Male Sprague Dawley Rats In Vivo: Inhibition by Angiotensin Converting Enzyme Inhibition (ACEi) Treatment. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3555. doi: https://doi.org/.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : High resolution MRI has documented retinal thickening in 3 mo diabetic male Sprague Dawley rats in vivo (IOVS,48:10, 4753). In this study, we characterized the time course of this supernormal retinal thickness and whether or not this thickening is normalized by ACEi treatment.

Methods: : In diabetic and age-matched nondiabetic male Sprague Dawley rats with and without treatment of the ACEi enalapril (mean range 5-7 mg/Kg, drinking water) central retinal thicknesses were measured from MRI (25 um) between 2 and 9 mo of diabetes. A subset of rats was chronically instrumented to allow for radiotelemetry readout of central blood pressure (BP) and heart rate (HR). Glycated hemoglobin and retinal thickness ex vivo (histology) were also collected.

Results: : Between 2 and 9 mo of diabetes, untreated rats had significantly (P < 0.05) thicker (avg. 15%) central retina in vivo than normoglycemic controls; thickening was not demonstrated on histology after fixation and dehydration. Enalapril normalized retinal thickness in vivo after 5 mo, but not 3 mo, of treatment. Relative to nondiabetic controls, HR and BP were subnormal in diabetic enalapril-treated rats, which were similar to diabetic rats.

Conclusions: : A surprisingly sustained retinal thickening in diabetic male Sprague Dawley rats in vivo was found on high resolution MRI examination; ACEi treatment corrected this supernormal thickness. Since our previous MRI data did not detect increase passive leakage through blood-retinal barrier until 8 mo of diabetes in this model, the present findings raise the possibility that the present retinal thickening is due, at least initially, to cytotoxic (as opposed to vasogenic) edema.

Keywords: edema • imaging methods (CT, FA, ICG, MRI, OCT, RTA, SLO, ultrasound) • diabetic retinopathy 
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