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Keijiro Ishikawa, Shigeo Yoshida, Yukio Sassa, Ryo Asato, Hiroaki Niiro, Koichi Akashi, Tatsuro Ishibashi, IV; Microarray Analysis of Fibrovascular Membranes from Patients with Proliferative Diabetic Retinopathy. Invest. Ophthalmol. Vis. Sci. 2011;52(14):3565.
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© ARVO (1962-2015); The Authors (2016-present)
Preretinal fibrovascular membranes (FVMs) form as a sequela to proliferative diabetic retinopathy (PDR) and can lead to severe decrease of vision. The factors controlling their progression and regression have not been fully determined. The purpose of this study was to determine a profile of gene expression in FVMs from patients with PDR.
Total RNA was isolated from human retina (Clontech) and FVMs obtained during vitrectomy from patients with PDR then used to generate double-stranded cDNA. Biotin-labeled cRNA was generated and hybridized to Illumina BeadChip Arrays. The fluorescence intensities of the scanned images were analyzed by computer and data mining was performed by Genespring software.
The levels of gene expression related to extracellular matrix, inflammation and stress response were up-regulated in FVMs compared to human retina. The levels of gene expression related to angiogenesis and cell differentiation were up-regulated in FVMs with active neovascularization (NV), whereas those related to fibrosis and apoptosis were up-regulated in FVMs with inactive NV.
The results suggest that some of the extracted genes may reflect the molecular mechanisms in the progression and regression of FVMs associated with PDR.
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