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Teruhiko Hamanaka, Akira Matsuda, Nobuo Ishida, Akira Murakami, Tetsuro Sakurai; D240 (Podoplanin) Immunohistochemical Staining Of The Trabecular Meshwork In Patients With Hereditary And Non-hereditary Primary Open Angle Glaucoma. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3232.
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To investigate D240 immunohistochemical staining reactivity (D240-IHSR) in the trabecular meshwork (TM), which may reflect TM abnormality in POAG (Watanabe et al. IOVS 51; 277, 2009)
Trabeculectomy (TLE) specimens obtained from 41 eyes of hereditary POAG (group A), 34 eyes of non-hereditary POAG (group B) and 11 eyes of NTG (group C) used as a control were embedded in paraffin and processed for D240 immunohistochemical staining. D240-IHSR in the juxta-canalicular meshwork (JCT), corneoscleral meshwork (CSM) and uveal meshwork (UVM) was divided into four grades (0-3). Grades of D240-IHSR and clinical parameters, age at TLE, max IOP before TLE, spherical equivalence (SE), C/D ratio and visual field impairment (Auhorn-Greve) were statistically evaluated among those groups.
Light microscopy observation did not show qualitative differences among three groups. No statistical difference in the grades of D240-IHSR in JCT was observed between group A (JCT: 0.73±0.81) and B (JCT: 1.00±0.69) (p=0.1215); however, grades of D240-IHSR in JCT were significantly lower in group A and B than in group C (2.36±0.50) (p=3x10-9). There was also a statistical difference in UVM between group A (2.44±0.87) and B (2.80±0.53) (p=0.02972). The grades of D240-ISHR in CSM of group A, B and C were 1.32±0.99, 1.83±0.95 and 2.64±0.50, respectively, the results revealing that D240-IHSR in CSM was statistically different among the three groups (p=2x10-6). Max IOP before TLE (group A: 30.88±8.87mmHg, group B: 26.74±5.94mmHg) and SE (group A: -4.13±4.60D, group B: -1.88±2.86D) showed significant differences between group A and B (max IOP: p=0.01826, SE: p=0.01149), but the other clinical parameters showed no statistical difference among those groups.
CSM and UVM abnormality is more involved in hereditary POAG than in non-hereditary POAG. This result may be the reason for higher max IOP before TLE in hereditary POAG. The morphological difference in TM between hereditary and non-hereditary POAG may be a quantitative abnormality in CSM and UVM.
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