March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Analysis Of Gene Expression In Acute Ischemic Neuroretinas: A Genome-wide Screen Discriminating Occlusion (BRVO) Versus Laser Effects In Rats
Author Affiliations & Notes
  • ALIKI GEKA
    Ophtalmology,
    Geneva University Hospital, Geneva, Switzerland
  • Aliki Geka
    Ophtalmology,
    Geneva University Hospital, Geneva, Switzerland
  • Cassandra Oropesa Ceballos
    Ophtalmology,
    Geneva University Hospital, Geneva, Switzerland
  • Angeliki Andrianaki
    Ophtalmology,
    Geneva University Hospital, Geneva, Switzerland
  • Odysseas Kargiotis
    Geneva University Hospital, Geneva, Switzerland
  • Olivier Schaad
    Faculty of Medecine, University of Geneva, Geneva, Switzerland
  • Alain Conti
    Ophtalmology,
    Geneva University Hospital, Geneva, Switzerland
  • Constantin J. Pournaras
    Ophtalmology,
    Geneva University Hospital, Geneva, Switzerland
  • Footnotes
    Commercial Relationships  ALIKI Geka, None; Aliki Geka, None; Cassandra Oropesa Ceballos, None; Angeliki Andrianaki, None; Odysseas Kargiotis, None; Olivier Schaad, None; Alain Conti, None; Constantin J. Pournaras, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3342. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      ALIKI GEKA, Aliki Geka, Cassandra Oropesa Ceballos, Angeliki Andrianaki, Odysseas Kargiotis, Olivier Schaad, Alain Conti, Constantin J. Pournaras; Analysis Of Gene Expression In Acute Ischemic Neuroretinas: A Genome-wide Screen Discriminating Occlusion (BRVO) Versus Laser Effects In Rats. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3342.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : Identification of genes differentially expressed in rat neuroretinas submitted to experimental acute branch retinal vein occlusion (BRVO), to laser treatment, or to light exposure.

Methods: : Using an in vivo experimental model of venous occlusion by photodynamic thrombosis in Long-Evans rat retinas, we induced acute ischemia by argon laser photocoagulation of venous sites adjacent to the optic nerve head in the right eye of one group of animals. In a second group, right eye retinas were exposed to laser treatment at sites located between major vessels. Finally, a third group of animals had their right eyes exposed to light through a slit lamp. Untreated left eyes served as controls in each animal. Total RNA was extracted from neuroretinas, 30 minutes and 6 hours post treatments, and processed for global gene analysis with Affymetrix microarrays. Genome-wide comparison of transcriptomes was then performed.

Results: : At 30 minutes, data did not reveal any sequence differentially expressed for the 3 treated groups. At 6 hours, light exposure was definitively excluded as a factor impacting gene expression. However, the expression of 627 and 113 sequences changed, respectively post BRVO and post laser treatment. When comparing transcriptomes of both groups with controls, we identified that the expression profiles of, respectively, 396 and 21 genes were specifically modified. Interestingly, around 80 genes, all upregulated, were common to both treated groups.The majority of differentially regulated genes encode proteins involved in different aspects of a large number of complex pathways, among which we retained: early response to stress, inflammation, response to hypoxia, angiogenesis, apoptosis and neuroprotection.

Conclusions: : Our microarray analysis revealed changes in gene expression bearing similarities to gene expression results from other ischemia models. Furthermore, it revealed that laser treatment may have an unreported and specific impact on retina’s metabolism.

Keywords: gene screening • retina • gene microarray 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×