March 2012
Volume 53, Issue 14
ARVO Annual Meeting Abstract  |   March 2012
Retinyl Ester Accumulation In The Retinal Pigment Epithelium In The Absence Of Light
Author Affiliations & Notes
  • Colleen K. Sheridan
    Biology, College Of Charleston, Charleston, South Carolina
  • Yiannis Koutalos
    Ophthalmology, Medical Univ of South Carolina, Charleston, South Carolina
  • Footnotes
    Commercial Relationships  Colleen K. Sheridan, None; Yiannis Koutalos, None
  • Footnotes
    Support  NIH/NEI Grant EY014850 and an unrestricted grant to MUSC Storm Eye Institute from Research to Prevent Blindness, Inc.
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3353. doi:
  • Views
  • Share
  • Tools
    • Alerts
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Colleen K. Sheridan, Yiannis Koutalos; Retinyl Ester Accumulation In The Retinal Pigment Epithelium In The Absence Of Light. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3353.

      Download citation file:

      © ARVO (1962-2015); The Authors (2016-present)

  • Supplements

Purpose: : To test whether the amount of retinyl esters in the Retinal Pigment Epithelium (RPE) increases in the absence of light. In the light, retinyl esters are mobilized as substrates for regeneration of the visual chromophore, 11-cis retinal. In mice lacking Rpe65, a protein required for regeneration of 11-cis retinal, large quantities of retinyl esters accumulate in the RPE.

Methods: : Wild-type mice (129/sv) were age matched and reared in the dark for various lengths of time over the course of one month. Whole eyecups were collected and retinyl esters were extracted and quantified using normal phase HPLC coupled to UV/Vis spectroscopy. The quantity of retinyl esters found in dark reared wild-type mice was compared to that in cyclic light reared and Rpe65-deficient mice.

Results: : The amounts of esters in the RPE of mice kept in the dark for up to 29 days was not significantly different from those in cyclic-light reared mice. Ester levels stayed relatively stable at ~50 pmol per eye in both dark- and cyclic-light reared mice. Quantities of retinyl esters in both groups of wild-type mice were much lower than in age-matched Rpe65-deficient mice, which were ~460 pmol/eye on Day 1 and ~1140 pmol/eye on Day 28.

Conclusions: : The stability of retinyl ester content over one month suggests that formation and storage of retinyl esters is tightly regulated in the RPE. The data suggest that Rpe65 is not simply involved in the generation of 11-cis retinal, but also in the regulation of the size of the retinyl ester pool.

Keywords: retinoids/retinoid binding proteins • retinal pigment epithelium • lipids 

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.