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Raffaele Parrozzani, Laura Bonaldi, Chiara Menin, Elisabetta Pilotto, Edoardo Midena; Fluorescence in-situ Hybridization vs Multiplex Ligation-dependent Probe Amplification for Uveal Melanoma Prognostication: in-vivo Comparative Results. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3398.
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To compare fluorescence in-situ hybridization (FISH) and multiplex ligation-dependent probe amplification (MLPA) for uveal melanoma prognostication.
Twenty-four patients affected by posterior uveal melanoma and scheduled for I125 brachytherapy were included in this study. Patients underwent in-vivo 25-G transcleral FNAB just before applying the radioactive plaque. Sampled material underwent both FISH and MLPA analysis using standard procedures. Follow-up was longer than 24 months.
Follow-up was 31±8 months (range, 25-42 months). FISH analysis revealed monosomy 3 in twelve cases (50%). MLPA revealed monosomy 3 in thirteen cases (54%) and a 3p14-q29 deletion in one case (4%) (classified as monosomy 3 by FISH). Nine patients (41%) developed metastatic disease during follow-up, including the case showing monosomy 3 only by MLPA. Patient with partial chromosome 3 deletion is still alive without metastases. Prediction of metastases based on monosomy 3 was higher for MLPA compared with FISH (p=0.008 vs p=0.035).
MLPA has a higher predictive value compared than FISH in UM prognostication based on monosomy 3 alone. The biological and prognostic value of partial chromosome 3 deletion, as well as others subtle chromosomes alterations or complex MLPA results, remains unclear.
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