March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Vision-guided Ocular Growth In A Mutant Chicken Model With Diminished Visual Acuity
Author Affiliations & Notes
  • Eric R. Ritchey
    College of Optometry,
    The Ohio State University, Columbus, Ohio
  • Christopher Zelinka
    Department of Neuroscience,
    The Ohio State University, Columbus, Ohio
  • Simon M. Petersen-Jones
    Small Animal Clinical Sciences, Michigan State University, East Lansing, Michigan
  • Andy J. Fischer
    Department of Neuroscience,
    The Ohio State University, Columbus, Ohio
  • Footnotes
    Commercial Relationships  Eric R. Ritchey, Vistakon (E); Christopher Zelinka, None; Simon M. Petersen-Jones, None; Andy J. Fischer, None
  • Footnotes
    Support  NIH Grant K12EY015447
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3446. doi:
  • Views
  • Share
  • Tools
    • Alerts
      ×
      This feature is available to authenticated users only.
      Sign In or Create an Account ×
    • Get Citation

      Eric R. Ritchey, Christopher Zelinka, Simon M. Petersen-Jones, Andy J. Fischer; Vision-guided Ocular Growth In A Mutant Chicken Model With Diminished Visual Acuity. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3446.

      Download citation file:


      © ARVO (1962-2015); The Authors (2016-present)

      ×
  • Supplements
Abstract

Purpose: : The proper regulation of ocular growth has been demonstrated to be a vision-dependent phenomenon across multiple species. We test whether vision-guided ocular growth is disrupted in an animal with a congenital defect causing diminished visual acuity.

Methods: : We examine vision-guided ocular growth in the Retinopathy, Globe Enlarged (RGE) chicken, which develops an ocular phenotype, including poor visual acuity in young animals without significant retinal abnormalities, resulting from a single codon deletion within guanine nucleotide-binding protein beta-3 (GNB3) (Montiani-Ferreira et al. 2003; Tummala et al. 2006). We investigate whether interrupted form-deprivation and lens-imposed defocus influence rates of ocular growth and retinal amacrine cells in RGE eyes.

Results: : We found that minus lens-wear increased rates of ocular growth in RGE chicks in a manner similar to that seen in wild-type (WT) chicks. Brief daily periods of normal vision interrupting form-deprivation prevented ocular enlargement in RGE chicks in a manner similar to that seen in WT chicks. However, the growth-slowing effects of plus lens-wear were diminished in RGE eyes compared to those seen in WT eyes. We found that glucagonergic amacrine cells differentially expressed Egr1 in response to growth-regulating visual stimuli in RGE retinas similar to expression patterns seen in WT retinas, with the exception of diminished responses to short-term treatment with plus-defocus. In addition, we provide evidence that the GNB3-positive bipolar cells could form synaptic connections with the glucagonergic amacrine cells.

Conclusions: : We conclude that the normal functions of photoreceptors and ON-bipolar cells in RGE retinas are not required for the growth-enhancing effects of minus lens-wear. The diminished responses of RGE eyes to plus-defocus may result from signaling deficits in photoreceptors and ON-bipolar cells.

Keywords: myopia • refractive error development • bipolar cells 
×
×

This PDF is available to Subscribers Only

Sign in or purchase a subscription to access this content. ×

You must be signed into an individual account to use this feature.

×