March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Visual Arrestins Influence Refraction and Ocular Biometry
Author Affiliations & Notes
  • Christopher C. Tan
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia
  • Han na Park
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia
  • B M. Brown
    Doheny Eye Institute and Department of Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, California
  • Jane Abey
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia
  • P M. Iuvone
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia
  • Cheryl Craft
    Doheny Eye Institute and Department of Ophthalmology, Keck School of Medicine of the University of Southern California, Los Angeles, California
  • Machelle T. Pardue
    Department of Ophthalmology, Emory University School of Medicine, Atlanta, Georgia
    Rehab R & D Center of Excellence, Atlanta Veterans Affairs Medical Center, Decatur, Georgia
  • Footnotes
    Commercial Relationships  Christopher C. Tan, None; Han na Park, None; B. M. Brown, None; Jane Abey, None; P. M. Iuvone, None; Cheryl Craft, None; Machelle T. Pardue, None
  • Footnotes
    Support  R01EY016435 (MTP) & Dept. of Veterans Affairs (MTP), R01EY015851 (CMC), P30EY03040 (DEI core), Fight for Sight (E-J.L), R01EY004864 (PMI), P30EY006360 (PMI), Research to Prevent Blindness.
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3450. doi:
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    • Get Citation

      Christopher C. Tan, Han na Park, B M. Brown, Jane Abey, P M. Iuvone, Cheryl Craft, Machelle T. Pardue; Visual Arrestins Influence Refraction and Ocular Biometry. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3450.

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      © ARVO (1962-2015); The Authors (2016-present)

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Abstract

Purpose: : Mice have two visual arrestins, Arr1, which is expressed in rods, cones and pineal, and Arr4, which is cone and pineal specific. Arrestins are involved in desensitization and recovery of G protein-coupled receptor activity, including opsins. Arr1 is needed for normal light adaptation (Brown et al., IOVS 2010;51:2372), while Arr4 is needed for contrast sensitivity (Brown et al., ARVO 2012). Based on the novel roles of visual arrestins in regulating visual responses, we examined refractive error and ocular biometry in Arr1-/-, Arr4-/-, and Arr1-/-Arr4-/- (DKO) mice and investigated the potential involvement of dopamine (DA) (Craft et al., ARVO 2012).

Methods: : Refraction and corneal radius of curvature (CRC) were measured with automated photorefractor and keratometer at postnatal day 60-70, respectively. Ocular biometry of anterior chamber depth (ACD), posterior chamber depth (PCD), corneal thickness (CT), retinal thickness (RT), lens thickness (LT), and axial length (AL) were collected from 1310nm spectral-domain optical coherence tomography images. Mice were dark-reared and retinas were collected under light and dark conditions to determine dopamine levels by high-performance liquid chromatography with coulometric detection.

Results: : Arrestin knockouts were significantly less hyperopic than wild-type (WT) controls. Arr1-/- and DKOs had significantly shorter AL and smaller LT. DKO mice had significantly steeper CRC and deeper ACD. The absence of Arr1 or Arr4 resulted in smaller CT, but DKOs were similar to WT. DA levels were similar between light and dark conditions in all genotypes. Arr1-/- mice had significantly higher DA levels than all other genotypes.

Conclusions: : DA levels in arrestin knockout mice did not show a consistent relationship to refractive or ocular parameters. However, the absence of both Arr1 and Arr4 influenced anterior chamber development. It is unclear if these changes are due to the alterations in visual regulation and contrast sensitivity in the absence of arrestins (Brown et al., ARVO 2012) or if the expression loss of arrestin proteins has a direct role on regulating eye size.

Keywords: retina • visual development • inner retina dysfunction: biochemistry and cell biology 
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