March 2012
Volume 53, Issue 14
Free
ARVO Annual Meeting Abstract  |   March 2012
Role of Human Corneal Stroma-Derived Mesenchymal-Like Stem Cells in Immunity, Wound Healing and Angiogenesis
Author Affiliations & Notes
  • Reka Albert
    Department of Biochemistry and Molecular Biology,
    Department of Ophthalmology,
    University of Debrecen, Debrecen, Hungary
  • Zoltan J. Vereb
    Department of Immunology,
    University of Debrecen, Debrecen, Hungary
  • Morten C. Moe
    Dept of Ophthalmology, Oslo University Hospital, Oslo, Norway
  • Laszlo Fesus
    Department of Biochemistry and Molecular Biology,
    University of Debrecen, Debrecen, Hungary
  • Eva Rajnavolgyi
    Department of Immunology,
    University of Debrecen, Debrecen, Hungary
  • Andrea Facsko
    Department of Ophthalmology, University of Szeged, Szeged, Hungary
  • Andras Berta
    Department of Ophthalmology,
    University of Debrecen, Debrecen, Hungary
  • Goran Petrovski
    Department of Biochemistry and Molecular Biology,
    Department of Ophthalmology,
    University of Debrecen, Debrecen, Hungary
  • Footnotes
    Commercial Relationships  Reka Albert, None; Zoltan J. Vereb, None; Morten C. Moe, None; Laszlo Fesus, None; Eva Rajnavolgyi, None; Andrea Facsko, None; Andras Berta, None; Goran Petrovski, None
  • Footnotes
    Support  None
Investigative Ophthalmology & Visual Science March 2012, Vol.53, 3503. doi:
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      Reka Albert, Zoltan J. Vereb, Morten C. Moe, Laszlo Fesus, Eva Rajnavolgyi, Andrea Facsko, Andras Berta, Goran Petrovski; Role of Human Corneal Stroma-Derived Mesenchymal-Like Stem Cells in Immunity, Wound Healing and Angiogenesis. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3503.

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Abstract

Purpose: : Mesenchymal stem cells (MSCs) can be isolated from several tissues including the cornea. Our goal was to establish in vitro assay for studying human corneal stroma injuries and the role stroma-derived stem cells have on immunity, wound healing and neovascularization processes.

Methods: : Human corneal buttons were harvested from cadavers (tissue collection complied with the guidelines of the Helsinki Declaration and was approved by the Regional Ethical Committee). Following removal of the epithelial and endothelial layers, stromal cells were proliferated in vitro on cell culture plate in medium containing human serum as the only supplement. To confirm MSC-like phenotype, FACS and gene array analysis, immunofluorescent staining and standardized in vitro differentiation assays were used. Functional tests including mixed lymphocyte response, wound healing and vascular tube-formation were also performed.

Results: : Corneal stroma cells could grow in culture for more than 10 passages (n=6). The most important markers of MSCs (CD73, CD90, CD105, CD 44, CD147, PDGFRb) were highly expressed, and no endothelial or hematopoietic cell markers were present on their surface. Differentiation into adipogenic, chondrogenic and osteogenic lineages were successful. Corneal stroma MSC-like cells could suppress the proliferation of activated peripheral blood lymphocytes, migrated and closed wounds within 24 hrs in vitro and formed vascular tube-like structures spontaneously within 8 hrs on Matrigel.

Conclusions: : We demonstrate an animal material free technique for cultivating MSC-like cells obtained from human corneal stroma. Their role in immunity, wound healing and angiogenesis could be studied in vitro, possibly serving as a model for examining corneal stroma injuries and their treatment accordingly.

Keywords: cornea: stroma and keratocytes • wound healing 
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