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Gorka Orive, Ignacio Alcalde, Francisco Muruzabal, Maria de la Fuente, Ana Fernandez, Jesus Merayo-Lloves, Eduardo Anitua; Plasma Rich in Growth Factors (PRGF-Endoret) accelerates corneal wound healing after PRK eye surgery, reducing haze formation. Invest. Ophthalmol. Vis. Sci. 2012;53(14):3537.
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To evaluate the efficacy of Plasma Rich in Growth Factors (PRGF-Endoret), an autologous platelet rich plasma technology, on the corneal wound healing process after Photorefractive Keratectomy (PRK) eye surgery.
Blood from healthy donors was collected, centrifuged and Plasma Rich in Growth Factors (PRGF-Endoret) was prepared avoiding the buffy coat. The effects of PRGF-Endoret treatment on the proliferation and migration of human corneal epithelial cells (HCE) was analyzed. PRK was performed on fifty black C57BL/6 mice distributed into two groups: one group was treated with PRGF-Endoret topical drops, and the other group was treated with saline serum. Corneal wound healing evolution was evaluated macroscopically with a surgical microscope and fluorescein staining. Eyes were collected after 0, 24, 48 and 72 hours, and after 7 days from the procedure, and were processed for histological studies. Immunohistochemistry was used to assess cellular proliferation, apoptosis and myofibroblasts transformation in the mouse cornea.
Proliferation rate of epithelial cells stimulated with PRGF-Endoret increased significantly when compared with non-treated group. Results from the in vitro wound healing study revealed that experimental area of 8.1 mm2 was totally closed by the epithelial cells (100% of the damage area) in 24 hours after treatment with PRGF-Endoret, while the non-treated control group was only partially closed (35% of total area). Mouse corneas treated with the autologous biological therapy after PRK showed a completely closure of the epithelial wounds significantly before than the control group. Futhermore, a reduction of haze formation was also observed. Histological studies showed a significant increase in the proliferative activity of corneal epithelial cells in PRGF-Endoret-treated group after PRK. The number of SMA-positive cells was significantly lower in the PRGF-Endoret group than in the control group, correlating with the results observed macroscopically.
These results suggest that PRGF-Endoret could accelerate corneal epithelial wound healing after PRK and reduce haze formation.
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