Effects Of The Ingredients, But Not Preservatives, Of Prostaglandin Analog Anti-glaucoma Eyedrops On Corneal Epithelial Wound Healing

Abstract

Purpose: : To examine the effects of the ingredients of prostaglandin (PG) analog anti-glaucoma eyedrops on behaviors of cultured SV40-immortalized human corneal epithelial cells (HCECs). The purpose of the current study also included comparing the effects of these ingredients on the cells to our previous results (ARVO 2011) of those by anti-glaucoma eyedrops with preservatives.

Methods: : We used these PG analog ingredients; latanoprost (Cayman); travoprost (Sigma); tafluprost (Santen); bimatoprost (Biomol). (1) Corneal blocks (2 x 4mm) obtained from an albino rabbit cornea were organ-cultured for 24h with or without these ingredients. (2) Alamar blue assay evaluated the effects these ingredients on HCEC proliferation. The dilution of each ingredient was adjusted to X100 or X1000 dilution of the commercially available eyedrop that contain each ingredient.

Results: : (1) Travoprost and tafluprost enhanced (p < 0.05) and bimatoprost inhibited (p < 0.05) corneal epithelial spreading compared to control. (2) Each PG analog ingredient increased cell proliferation (p < 0.01).

Conclusions: : The effects of ingredients of PG analog anti-glaucoma eyedrops varies among each component; Travoprost and tafluprost accelerated and bimatoprost suppressed epithelial spreading in organ-cultured cornea. That is a similar to our previous results (ARVO 2011) with commercially available eyedrops at the same dilution. The ingredients alone promoted cell proliferation, different from our previous results (ARVO 2011) that showed each eyedrop did not alter cell proliferation.

Keywords: cornea: epithelium • wound healing